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04743814001 Roche

Expand 20 kbPLUS PCR System, dNTPack

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Properties

usage   sufficient for ≤40 reactions
packaging   pkg of 200 U
mfr. no.   Roche
storage condition   avoid repeated freeze/thaw cycles
parameter   68 °C optimum reaction temp.
shipped in   dry ice
storage temp.   −20°C (−15°C to −25°C)

Description

General description

The Expand 20 kbPLUS PCR System is composed of an enzyme mix containing thermostable Taq DNA polymerase and Tgo DNA polymerase, a thermostable DNA polymerase with proofreading activity. This powerful polymerase mixture and associated buffer system is designed to give a high yield of PCR product when fragments longer than 20 kb need to be amplified.
T/A cloning can also be performed.

The kit has control human genomic DNA and a set of control primers for amplifying a 23kb fragment. This test can be used to test template DNA and primer pair performance.
Use 3.75 U for a standard 50μL PCR.
Each dNTPack contains 10mM additive-free sodium salt nucleotides as a ready-to-use mix.
To obtain long PCR products, first evaluate length and purity of the DNA template. Handling all genomic DNA templates with care. Genomic DNA template should be larger than 50kb, as determined with agarose gel electrophoresis.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Application

Choose Expand 20 kbPLUS PCR System when amplifying genomic DNA fragments up to 35 kb. Expand 20 kbPLUS PCR System features a specifically optimized buffer and enzyme-blend mixture to amplify extra-long pieces of DNA (over 20 kb). Control primers and DNA produce fragments of 23 kb in length.
• PCR
• RT-PCR
• Large-fragment amplification

Features and Benefits

• Go the limit: The buffer and enzyme-blend amplify products over 20kb DNA.
• Easy PCR monitoring: Human genomic DNA and human control β-globin primers amplify a 23kb fragment.
• Test template quality: Control reagents also test template quality and primer pair performance.
• Cost-effective: Use the convenient premixed solution of PCR grade dNTPs.

Packaging

1 kit containing 7 components

Quality

Each lot of Expand 20kbPLUS PCR System is function-tested in PCR using human genomic DNA and specific primers for β-globin to amplify a 23kb fragment.

Unit Definition

Volume Activity: 5 U/μl

Storage and Stability

Store supplied human control DNA at 2–8 °C, all other components at -15 to -25°C.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Expand is a trademark of Roche

Kit component only

Description

Product #

Add to Cart

Expand 20 kbPLUS Enzyme Mix    
Expand 20 kbPLUS Reaction Buffer, with 27.5 mM MgCl2 10x concentrated    
MgCl2 Stock Solution 25 mM    
Human Genomic DNA    
Human β-globin Control Primer forward, (HβG forward)    
Human β-globin Control Primer reverse, (HβG reverse)    
PCR Nucleotide Mix    
Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash
Protocols & Articles

Articles

Hot Start PCR

Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot Start PCR allows fo...
Keywords: Amplification, Buffers, Gas chromatography, Polymerase chain reaction, Polymerase chain reaction - quantitative

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