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10109118001 Roche

Reverse Transcriptase AMV

Synonym: amv reverse transcriptase

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Properties

Related Categories Molecular Biology, PCR/Amplification, RT-PCR and RT-qPCR More...
Quality Level   100
form   solution
specific activity activity   >50 units/μg protein
packaging   pkg of 1,000 U
mfr. no.   Roche
storage condition   avoid repeated freeze/thaw cycles
parameter   42 °C optimum reaction temp.
shipped in   dry ice

Description

General description

Reverse Transcriptase, AMV is a gene product of the RNA genome of avian myeloblastosis virus. The enzymatically active forms of the purified enzyme are α,ββ and αβ. The molecular weight of the α-subunit is 68 kDa, that of the β-subunit 92 kDa. The mature αβ form, the most active form of Reverse Transcriptase, AMV, includes a RNA-directed DNA polymerase, a DNA-dependent DNA polymerase, a RNase H, and an unwinding activity. Reverse Transcriptase, AMV is used for cDNA synthesis, for synthesis of first strand cDNA for use in subsequent amplification reactions and dideoxy DNA sequencing.
The enzyme can also be used for RNA sequencing, 3′ end labeling of DNA fragments, and the generation of ss probes for genomic footprints.

Reverse Transcriptase AMV requires a primer and Mg2+ or Mn2+ for activity.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Specificity

AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is an RNA-directed DNA polymerase and a DNA-dependent DNA polymerase. The AMV reverse transcriptase requires a primer and Mg2+ or Mn2+ for activity.
Heat inactivation: 5 min, 95 °C

Application

Reverse Transcriptase AMV is suitable for:
• First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
• Dideoxy DNA sequencing
• Primer extension
• RNA sequencing
• 3′-end labeling of DNA fragments
• Generation of single-stranded probes for genomic footprint experiments

Features and Benefits

• Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
• Procure full length cDNA fragments up to 12 kb
• Higher thermostability (up to 60°C) and specificity than M-MuLV Reverse Transcriptase

Packaging

1 kit containing 2 components

Quality

Absence of contaminants: Tested for the absence of detectable nonspecific RNases and nonspecific DNases in incubations with various nucleic acids (analyzed by gel electrophoresis) according to the current Quality Control procedures.
Function test: Reverse Transcriptase AMV is function tested in the cDNA Synthesis Kit and RT-PCR.

Unit Definition

1 unit is the enzyme activity that incorporates 1 nmol of [3H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)+ · d(pT)15 as template primer.

Volume Activity: 20-25 U/μl. Please refer to the Certificate of Analysis for more information.

Preparation Note

Storage buffer: 200 mM potassium phosphate, 2 mM dithiothreitol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), pH 7.2.

Storage and Stability

Store at -15–-25 °C. (Storage at -70 °C is more likely to deteriorate the enzyme via repeated freezing and thawing.)

Kit component only

Description

Product #

Add to Cart

Reverse Transcriptase AMV 20-25 U/μl    
First-strand cDNA Synthesis Buffer 5x concentrated    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash
Protocols & Articles

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Peer-Reviewed Papers
15

References

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