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11855476001 Roche

Titan One Tube RT-PCR System

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Properties

Related Categories Molecular Biology, PCR/Amplification, RT-PCR and RT-qPCR More...
Quality Level   100
usage   sufficient for 100 reactions
mfr. no.   Roche
parameter   42-60 °C optimum reaction temp.
shipped in   dry ice
storage temp.   −20°C

Description

General description

The Titan One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:
• Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
• Delivers increased sensitivity due to the high efficiency of all three enzymes.
• Is faster than conventional two-step RT-PCR.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Specificity

Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV

Application

Titan One Tube RT-PCR System has been used:
• to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNA
• for determining the presence or absence of RNA templates
• in cloning of rare messages without the need to construct cDNA libraries
• for quantifying the levels of gene expression

Features and Benefits

Titan One Tube RT-PCR System:
• High Fidelity: Expand High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR)
• offers a threefold higher fidelity in comparison to Taq DNA polymerase
• delivers increased sensitivity due to the high efficiency of all three enzymes
• faster than conventional two-step reverse transcriptase (RT)-PCR
• allows the amplification of full-length cDNA up to 6 kb by RT-PCR due to the use of the Expand PCR System

Packaging

1 kit containing 4 components

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Expand is a trademark of Roche

Titan is a trademark of Roche

Kit component only

Description

Product #

Add to Cart

Reverse Transcriptase AMV and Expand High Fidelity Enzyme Mix in storage buffer    
RT-PCR Reaction Buffer 5x concentrated    
MgCl2 Stock Solution, 25 mM    
DTT Solution, 100 mM    
Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Flash Point(F) 
does not flash
Flash Point(C) 
does not flash

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Coronavirus qPCR Design Case Study & Related Products to Support SARS-CoV-2 Research

We are working hand in hand with public health authorities and customers to support the fight against novel coronavirus (SARS-CoV-2). In response to the community’s need for SARS-CoV-2-specific reage...
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Titan One Tube RT-PCR System Protocol & Troubleshooting

Amount of poly(A) RNA template For best results, start with 1 ng - 100 ng poly(A)-RNA. Template preparation The required template RNA can be isolated using the mRNA Capture Kit or the mRNA Isolation ...
Keywords: Amplification, Gene expression, Nucleic acid denaturation, Obesity, Polymerase chain reaction, Respiratory

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PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
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RT-PCR | Reverse Transcription PCR

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Peer-Reviewed Papers
15

References

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