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2GFMPXKB Roche

KAPA2G Fast Multiplex Mix

  •  NACRES NA.55

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Properties

Quality Level   100
shelf life   ≤12 mo.
packaging   kit of 1.25 mL (100 x 25 μL rxn; KK5801)
  kit of 6.25 mL (500 x 25 μL rxn; KK5802)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

General description

KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzyme derived through a process of directed evolution. KAPA2G FAST HotStart DNA Polymerase is an antibody-mediated hot start formulation engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes, allowing for more uniform multiplexed PCR.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Application

KAPA2G Fast Multiplex Mix has been used for:
• Typing of transgenic organisms
• Amplification of microsatellites
• Typing and detection of pathogens
• Amplification of multiple DNA fragments for SNP genotyping

Features and Benefits

Improve sensitivity, specificity, and yields
• Uniform representation of all amplicons
• Successful multiplex PCR with difficult, GC-rich targets

Increase speed without compromising performance
• 60% reduction in cycling time
• Extension times as low as 15 seconds

Quick Notes:
• KAPA2G Fast Multiplex Mix contains the engineered KAPA2G Fast HotStart DNA Polymerase, for fastand efficient multiplex PCR.
• The KAPA2G Fast Multiplex Mix contains a buffer optimized for multiplex PCR, with 0.2 mM of each dNTP and 3 mM MgCl2 (at 1X).
• Use 0.2 μM of each primer, and 10–100 ng of template DNA per reaction.
• Anneal at 60°C for 30 seconds.
• Perform extension for 15 sec, and increase for longer amplicons, and/or highly multiplexed reactions.

Packaging

Kits include KAPA2G Fast Multiplex Mix (2X) which contains KAPA2G Fast HotStart DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 3 mM).

Quality

Each batch of KAPA2G Fast HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (AgilentProtein 230 Assay). KAPA2G Fast Multiplex PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contaminationlevels.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the ReadyMix has been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user’s own risk.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Directed Evolution

Proteins, the functional workhorses of living organisms, are composed of chains of interlinked amino acids that together form peptides and ultimately dictate the three-dimensional structure and funct...

KAPA2G Fast Multiplex Kits

For which applications should I use KAPA2G Fast Multiplex PCR Kit? The KAPA2G Fast Multiplex PCR Kit is ideally suited for end-point, fast multiplex PCR of multiple DNA fragments, ranging in size fro...
Keywords: Amplification, Capillary electrophoresis, Electrophoresis, Gas chromatography, Melting, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

Kapa Biosystems Taq DNA Polymerase and PCR Kits

DNA extraction and amplification are critical steps in your molecular biology workflow and often requires purified, high-quality DNA. Kapa Biosystems offers a robust portfolio of DNA polymerase reage...
Keywords: Amplification, Centrifugation, Cloning, DNA purification, Gas chromatography, Genetic, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Polymerase chain reaction, Purification, Reductions, Sample preparations, Sequencing, Size-exclusion chromatography, Solvents

Peer-Reviewed Papers
15

References

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