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KAPA2G Fast Multiplex Mix

  •  NACRES NA.55



Quality Level   100
shelf life   ≤12 mo.
packaging   kit of 1.25 mL (100 x 25 μL rxn; KK5801)
  kit of 6.25 mL (500 x 25 μL rxn; KK5802)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C


General description

KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzyme derived through a process of directed evolution. KAPA2G FAST HotStart DNA Polymerase is an antibody-mediated hot start formulation engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes, allowing for more uniform multiplexed PCR.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.


KAPA2G Fast Multiplex Mix has been used for:
• Typing of transgenic organisms
• Amplification of microsatellites
• Typing and detection of pathogens
• Amplification of multiple DNA fragments for SNP genotyping

Features and Benefits

Improve sensitivity, specificity, and yields
• Uniform representation of all amplicons
• Successful multiplex PCR with difficult, GC-rich targets

Increase speed without compromising performance
• 60% reduction in cycling time
• Extension times as low as 15 seconds

Quick Notes:
• KAPA2G Fast Multiplex Mix contains the engineered KAPA2G Fast HotStart DNA Polymerase, for fastand efficient multiplex PCR.
• The KAPA2G Fast Multiplex Mix contains a buffer optimized for multiplex PCR, with 0.2 mM of each dNTP and 3 mM MgCl2 (at 1X).
• Use 0.2 μM of each primer, and 10–100 ng of template DNA per reaction.
• Anneal at 60°C for 30 seconds.
• Perform extension for 15 sec, and increase for longer amplicons, and/or highly multiplexed reactions.


Kits include KAPA2G Fast Multiplex Mix (2X) which contains KAPA2G Fast HotStart DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 3 mM).


Each batch of KAPA2G Fast HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (AgilentProtein 230 Assay). KAPA2G Fast Multiplex PCR Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contaminationlevels.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the ReadyMix has been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user’s own risk.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Directed Evolution

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