• USA Home
  • TAQNL-RO - Taq DNA Polymerase (1 U/μl), dNTPack

EMAIL THIS PAGE TO A FRIEND
TAQNL-RO Roche

Taq DNA Polymerase (1 U/μl), dNTPack

Purchase

Properties

Related Categories Molecular Biology, PCR/Amplification, Routine PCR Amplification More...
Quality Level   100
usage   sufficient for ≤2,000 reactions (04738241001)
  sufficient for ≤500 reactions (04738225001)
packaging   pkg of 1,000 U (04738241001 [4 x 250 U])
  pkg of 250 U (04738225001)
mfr. no.   Roche
parameter   72 °C optimum reaction temp.
optimum pH   ~9.0 (20 °C)
shipped in   dry ice
storage temp.   −20°C

Description

General description

Taq DNA Polymerase (1 U/μl), dNTPack, comprises Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. Taq DNA polymerase is a highly processive 5′-3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It is stable during prolonged incubations at elevated temperatures (+95 °C). Additionally, it exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. It accepts dNTP analogs as substrates. There is no difference in stability or performance when compared to the standard concentration of Taq DNA Polymerase.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Application

Taq DNA Polymerase (1 U/μl), dNTPack may be used for:
• PCR
• RT-PCR
• Other primer-extension reactions, such as sequencing and labeling

Packaging

1 kit containing 5 components

Unit Definition

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl2, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 1 U/μl

Kit component only

Description

Product #

Add to Cart

Taq DNA Polymerase    
PCR Buffer with MgCl<sub>2</sub> 10x concentrated    
MgCl<sub>2</sub> Stock Solution    
PCR Buffer without MgCl<sub>2</sub>    
PCR Nucleotide Mix</ul>    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?