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THSNTKB Roche

KAPA Taq HotStart

with dNTPs

  •  NACRES NA.55

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Properties

Quality Level   100
shelf life   ≤18 mo.
packaging   pkg of 250 U (KK1509)
  pkg of 500 U (KK1511)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

General description

KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

KAPA Taq HotStart with dNTPs is supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Application

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR

Features and Benefits

High performance
• Improved sensitivity, specificity, and yields.
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product.

Quick Notes:
• KAPA Taq HotStart DNA Polymerase can replace any commercial hotstart Taq DNA polymerase in an existing protocol. The final MgCl2 concentration and annealing temperature may need to be optimized to account for differences in formulation.
• The KAPA Taq HotStart Buffer is a uniquelyformulated buffer offering improved specificity and sensitivity, and improved amplification of GC- and AT-rich templates.
• The KAPA Taq HotStart Buffer does not contain MgCl2; MgCl2 (25 mM) is supplied separately to allow greater flexibility during reaction setup.
The KAPA Taq HotStart PCR Kit is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Packaging

KAPA Taq HotStart with dNTPs includes the following:
• KAPA Taq Standard or HotStart DNA Polymerase 5 U/μL
• 10X KAPA Taq Buffer A
• 10X KAPA Taq Buffer B
• 10X KAPA Buffer with loading dye (optional)
• 5X KAPA Taq HotStart Buffer (HotStart kits only)
• MgCl2 (25 mM)
• dNTP Mix (10 mM each; optional)

Quality

Each batch of KAPA Taq HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq HotStart PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long term storage.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Directed Evolution

Proteins, the functional workhorses of living organisms, are composed of chains of interlinked amino acids that together form peptides and ultimately dictate the three-dimensional structure and funct...

Kapa Biosystems Taq DNA Polymerase and PCR Kits

DNA extraction and amplification are critical steps in your molecular biology workflow and often requires purified, high-quality DNA. Kapa Biosystems offers a robust portfolio of DNA polymerase reage...
Keywords: Amplification, Centrifugation, Cloning, DNA purification, Gas chromatography, Genetic, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Polymerase chain reaction, Purification, Reductions, Sample preparations, Sequencing, Size-exclusion chromatography, Solvents

Peer-Reviewed Papers
15

References

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