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D5809 Sigma-Aldrich

JumpStart AccuTaq LA DNA Polymerase

Hot-start high fidelity Taq enzyme, 10X buffer included

Synonym: JumpStart® AccuTaq LA DNA Polymerase

  •  NACRES NA.55

Purchase

Properties

Related Categories Core Bioreagents, Hot Start PCR, JumpStart PCR Products, Life Science Reagents for PCR, Long & Accurate PCR,
Quality Level   200
form   liquid
feature   hotstart
concentration   2.5 unit/μL
color   colorless
  colorless
shipped in   wet ice
storage temp.   −20°C

Description

Other Notes

View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart.

Application

JumpStart AccuTaq LA DNA Polymerase has been used in polymerase chain reaction(PCR) for the amplification of soil DNA samples and in accurate long range PCR of histone H2A.Z in bacterial artificial chromosome (BACs).

JumpStart AccuTaq LA DNA Polymerase is a combination of AccuTaq LA DNA Polymerase and a Taq-directed antibody. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot start mechanism provides increased specificity and higher target yield in comparison to standard amplification. JumpStart AccuTaq LA DNA Polymerase can generate long products with higher fidelity (up to 6.5× of Taq DNA polymerase).

Features and Benefits

• JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• Up to 6.5× greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR
• Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
• Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods

Packaging

Supplied with 10× reaction buffer.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

Jacobs is a registered trademark of Jacobs

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Hot Start PCR

Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot Start PCR allows fo...
Keywords: Amplification, Buffers, Gas chromatography, Polymerase chain reaction, Polymerase chain reaction - quantitative

Protocols

End Point PCR Protocol for Long and Accurate DNA Amplification

Technology Overview Equipment Reagents Assay Considerations Procedure Troubleshooting Materials References
Keywords: Amplification, Buffers, Cloning, Digestions, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Reductions, Sequencing, Size-exclusion chromatography, transformation

Related Content

Long & Accurate PCR

Many polymerase chain reaction (PCR) applications require longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase. Conventional Taq DNA polymer...
Keywords: Amplification, Cloning, Gene expression, Polymerase chain reaction, Size-exclusion chromatography

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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