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D6442 Sigma-Aldrich

JumpStart Taq ReadyMix for High Throughput Quantitative PCR

Ready-to-use 2x mix for qPCR with ROX

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Properties

Related Categories Amplification, Cell Biology, Functional Profiling of Stem Cells, General Stem Cell Biology, Molecular Biology,
Quality Level   200
form   liquid
feature   hotstart
concentration   2.5 units/reaction (50 μL reaction volume)
color   colorless
Featured Industry   Agriculture
compatibility   for use with ABI 5700
  for use with ABI 7000
  for use with ABI 7300
  for use with ABI 7500 Fast
  for use with ABI 7500
  for use with ABI 7700
  for use with ABI 7900 Fast
  for use with ABI 7900 HT
  for use with ABI 7900
  for use with ABI StepOne
  for use with ABI StepOnePlus
  for use with ABI ViiA 7
  for use with Bio-Rad CFX384
  for use with Bio-Rad CFX96
  for use with Bio-Rad MJ Chromo4
  for use with Bio-Rad MJ Opticon 2
  for use with Bio-Rad MJ Opticon Cepheid SmartCycler
  for use with Bio-Rad MJ Opticon
  for use with Bio-Rad MiniOpticon
  for use with Eppendorf® Mastercycler ep realplex2 s
  for use with Eppendorf® Mastercycler ep realplex
  for use with Illumina Eco qPCR
  for use with Qiagen Corbett Rotor-Gene 3000
  for use with Qiagen Corbett Rotor-Gene 6000
  for use with Qiagen Corbett Rotor-Gene Q
  for use with Roche LightCycler 480
  for use with Strategene Mx3000P
  for use with Strategene Mx3005P
  for use with Strategene Mx4000
shipped in   wet ice
storage temp.   −20°C

Description

Other Notes

JumpStart Taq ReadyMix For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. The ReadyMix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. It is provided in a 2× concentrate. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.

JumpStart Taq ReadyMix for High Throughput Quantitative PCR is a reaction formulation used for high throughput hot-start quantitative PCR methods. It contains the hot start DNA polymerase reaction mixture with ROX dye as an internal reference for normalization of reactions.

Application

• For qPCR amplifications using SYBR
• For multiplex PCR
• For reduction of primer dimers

Features and Benefits

• To prepare a typical QPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL . Reaction volumes can be scaled down, if desired.
• JumpStart Taq DNA polymerase prevents non-specific product formation, and allows assembled PCR reactions to be placed at room temperature for up to two hours without compromising the performance.
• Internal Reference Dye is provided for reaction normalization.
• When performing large numbers of PCR reactions, JumpStart ReadyMix Taq can save a significant amount of preparation time, reduce the risk of contamination from multiple pipetting steps, and provide consistent batch-to-batch and reaction-to-reaction performance.

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

Quantity

Use 2.5 units per reaction.

Legal Information

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
Protocols & Articles

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Peer-Reviewed Papers
15

References

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