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MSRT2 Sigma-Aldrich

SigmaProt Intact Protein LC-MS Standard

Proteomics Retention Time Standard

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Properties

Related Categories Mass Spectrometry, Molecular Biology, Peptide and Protein Standards for Mass Spectrometry Analysis, Proteomics
Quality Level   200
storage temp.   −20°C

Description

Application

The RP-LC-MS Protein Standard is a LC-MS platform standard that is intended to test LC-MS system suitability and intact protein mass measurement. The proteins in MSRT2 are chosen to represent an elution profile for complex proteomic samples. The proteins have been selected based on UV and electrospray response, and are readily observed by both modes of detection. After reconstitution, MSRT2 is an injection-ready standard. MSRT2 can be utilized in studies such as in top-down proteomics.

Components

MSRT2 contains these 9 proteins:
• Ribonuclease B
• Insulin
• Lysozyme
• Transferrin
• Bovine serum albumin (BSA)
• Trypsin inhibitor
• β-lactoglobulin A
• Carbonic anhydrase
• Lactate dehydrogenase

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Charge State Dependent Fragmentation in Top-Down Analysis by RP-LC-MS/MS Using a Standard Protein Mix

Analytical labs that serve as a core facility are often tasked with performing LC-MS analysis of intact proteins from various internal and/or external research groups. The most widely used approaches...
Keywords: Digestions, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Proteomics, Reductions

UHPLC Analysis of Proteomics Retention Time Standard on BIOshell™ IgG 1000 Ã… C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Proteomics Retention Time Standard on BIOshell™ IgG 1000 Ã… C4
Keywords: Proteomics, Solid phase peptide synthesis

Related Content

Stable Isotope-labeled MS Internal Standards

What happens when you combine state-of-the-art technology with scientific prowess? Full-length protein standards with the highest purity and isotopic incorporation in the industry. Stable isotope-lab...
Keywords: Atomic absorption spectroscopy, Buffers, Digestions, Mass spectrometry, PAGE, Phosphorylations

Peer-Reviewed Papers
15

References

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MSRT1 MS RT Calibration Mix, Proteomics Retention Time Standard for LC-MS

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