• USA Home
  • QR0200 - Quantitative RT-PCR ReadyMix

EMAIL THIS PAGE TO A FRIEND
QR0200 Sigma-Aldrich

Quantitative RT-PCR ReadyMix

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

  •  NACRES NA.55

Purchase

Properties

Related Categories Molecular Biology, Nucleic Acid Amplification, PCR/Amplification, Plant Biotechnology, Plant Molecular Biology,
Quality Level   200
feature   hotstart
color   colorless
shipped in   wet ice
storage temp.   −20°C

Description

General description

Quantitative RT-PCR ReadyMix combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Application

Quantitative RT-PCR ReadyMix has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

Features and Benefits

• The master mix allows consistency and reproducibility from one reaction to the next
• Reduced risk of contamination from multiple pipetting steps
• Reduced set-up time as compared to manual or wax Hot Start methods
• JumpStartTaq Polymerase reduces primer-dimer and non-specific product formation
• Broad instrument compatibility
• Includes a separate ROX reference dye vial for reaction normalization

Packaging

1 kit sufficient for 250 reactions at 20 μL each

Legal Information

This product is for research use only.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit component also available separately

Description

Product #

Add to Cart

10X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 X SDS P2192
Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 25 mM SDS M8787
10X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 X SDS P2192

Kit component only

Description

Product #

Add to Cart

Probe Based qRT-PCR ReadyMix 2 X    
Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Betsy Boedeker, Gregory A. Wemhoff, Andrea Spencer,Roger Chiu, Suzanne Miller, Henry George and Edward J. Weinstein
LSI Volume 7 Article 1
Keywords: Amplification, Antibiotics, Cellular processes, Drug discovery, Functional genomics, Gene expression, Genomics, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Sequences, Transcription, Transduction, Vectors

Metagenomics and Quantitative PCR

The highly variable regions within the 16S ribosomal RNA (rRNA) gene sequences are being exploited with PCR and metagenomic sequencing to characterize the microbial strains within the gastrointestina...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Gastrointestinal, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Probiotics, Sequencing

Reverse Transcription - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Degradations, Gene expression, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription

Using probe-based quantitative PCR to measure gene-level expression

Quantitative PCR has reached a level of sensitivity, accuracy, and ease to support use as a routine assay for measuring gene-level expression. The field of cancer research is validating a number of a...
BioFiles Vol. 4, No. 7
Keywords: Amplification, Cancer, Clinical, Diagnostic, Gene expression, Genetic, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Sample preparations, Size-exclusion chromatography

Protocols

Coronavirus qPCR Design Case Study & Related Products to Support SARS-CoV-2 Research

We are working hand in hand with public health authorities and customers to support the fight against novel coronavirus (SARS-CoV-2). In response to the community’s need for SARS-CoV-2-specific reage...
Keywords: Amplification, Diagnostic, Gene expression, Genetic, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription, Transfection

Reverse Transcription Protocol (One-step Probe Detection)

In some cases it is preferable to measure the target transcript directly, without preparing cDNA from the entire RNA sample. Such situations may include measurements on highly degraded RNA or when th...
Keywords: Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

Related Content

Coronavirus COVID-19 (SARS-CoV-2) Detection, Characterization, Vaccine and Therapy Production

As a global life science supplier, we are here to help in the response to COVID-19. From supplying raw materials and research products for detection and characterization to production, we’ve consolid...
Keywords: PAGE

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?