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UPS2 Sigma-Aldrich

Proteomics Dynamic Range Standard Set

Protein Mass Spectrometry Calibration Standard

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Related Categories Mass Spectrometry, Molecular Biology, Peptide and Protein Standards for Mass Spectrometry Analysis, Proteomics
Quality Level   200
quality   Protein Mass Spectrometry Calibration Standard
concentration   10.6 μg/ampule protein
shipped in   wet ice
storage temp.   −20°C

Description

General description

The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.

Application

Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins, embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes. It has also been used as a standard to spike HeLa cells for label-free quantification.

The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation. It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.

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Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg SDS T6567
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Universal Proteomics Standards – Validating the Future of Proteomics

The era of high-throughput proteomics has recently blossomed due in large part to advances in the methods by which proteins and proteomes are analyzed. Improved fractionation techniques, combined wit...
LSI Issue 24
Keywords: Amino acid analysis, Fractionation, High performance liquid chromatography, Mass spectrometry, Methods, Newsletters, PAGE, Proteomics, Protocols, Purification, Search, Separation, Support

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Stable Isotope-labeled MS Internal Standards

What happens when you combine state-of-the-art technology with scientific prowess? Full-length protein standards with the highest purity and isotopic incorporation in the industry. Stable isotope-lab...
Keywords: Atomic absorption spectroscopy, Buffers, Digestions, Mass spectrometry, PAGE, Phosphorylations

Peer-Reviewed Papers
15

References

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UPS1 Universal Proteomics Standard Set, Protein Mass Spectrometry Calibration Standard
MSQC1 MS Qual/Quant QC Mix, Proteomics MRM LC-MS Calibration Standard Parent proteins of MSQC1 are all present in UPS2.
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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