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XNAB2R Sigma-Aldrich

Extract-N-Amp Blood PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

  •  NACRES NA.55



Related Categories Automation Reagents, Cell Biology, DNA & RNA Purification, General Stem Cell Biology, Genomic DNA Amplification,
Quality Level   200
usage   sufficient for 1000 amplifications
  sufficient for 1000 extractions
color   colorless
shipped in   wet ice
storage temp.   −20°C


General description

The Extract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR (Fig. 1). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.


Extract-N-Amp Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).

Features and Benefits

• Efficient 8 minute prep allows greater speed and throughput
• No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
• Simple, 3 step procedure with no special equipment required
• Hot start antibody included for highly specific PCR amplification of genomic DNA
• Compatible with multiple format (single tube or 96-well plate)
• Can be used with whole blood or blood cards
• Extract stable at 4 °C for at least 6 months (Fig. 2)


Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

UN 3316 9/PG 3
Protocols & Articles


Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. As shown in this article, this methodolog...
Mairi J. Hunter, Iain McLaren-Lee, Nicholas R. Helps
LSI Edition 23
Keywords: Amplification, Polymerase chain reaction, Polymorphisms, Sample preparations, Sequencing, Size-exclusion chromatography

Extract-N-Amp™ Blood Product Information

The Extract-N-Amp Blood PCR kits contain all of the reagents necessary to rapidly extract genomic DNA from whole blood and amplify targets of interest by PCR (Figure 1).
Keywords: Amplification, Centrifugation, Cloning, DNA purification, Digestions, Filtration, Polymerase chain reaction, Precipitation, Purification, Sequencing

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample puri...
Keywords: Amplification, Buffers, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing

Genomic DNA preparation kits | Genomic DNA Extraction | PCR Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy


Extract-N-Amp™ Blood PCR Kit Protocol

Product Description Reagents and Equipment Required But Not Provided Storage/Stability Precautions and Disclaimer Procedure PCR amplification Troubleshooting Guide Materials References
Keywords: Amplification, Coagulation, Detection methods, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Sequencing, Solvents

Related Content

Automated Protocols for Life Science Research

Our scientists have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply information such as i...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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