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MABD102 Sigma-Aldrich

Anti-Lin28 Antibody, clone PR3G10

Anti-Lin28 Antibody, clone PR3G10 is a highly specific mouse monoclonal antibody, that targets lin28 & has been tested in western blotting & ICC.

Synonym: Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, L-LL, Primary Antibodies
species reactivity   human
application(s)   immunocytochemistry: suitable
  western blot: suitable
clone   PR3G10, monoclonal
isotype   IgG1κ
shipped in   wet ice
Gene Information   human ... LIN28A(79727)
biological source   mouse
antibody product type   primary antibodies
NCBI accession no.   NP_078950
UniProt accession no.   Q9H9Z2

Description

Immunogen

Epitope: Near N-Terminus

Ovalbumin-conjugated linear peptide corresponding to human Lin28.

General description

Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Application

Research Category
Stem Cell Research

Research Sub Category
RNA Metabolism & Binding Proteins

Western Blot Analysis: 1 µg/mL from a representative lot detected Lin28 in human fetal brain tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution (2 µg/mL) from a representative lot detected Lin28 in mESCs.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Lin28 in 10 µg of human placenta tissue lysate.

Target description

~28 kDa observed

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human placenta tissue lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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