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XTG9-RO Roche

X-tremeGENE 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines

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Properties

Related Categories Application: For commonly used cells, Cell Biology, Cloning and Expression, Life Science Research, Molecular Biology,
Quality Level   100
grade   for molecular biology
form   liquid (aqueous solution)
usage    mL (suitable for 165 transfections)
packaging   pkg of 0.4 mL (06365779001)
  pkg of 1.0 mL (06365787001)
  pkg of 5 × 1.0 mL (06365809001)
mfr. no.   Roche
shipped in   wet ice
storage temp.   2-8°C

Description

General description

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Application

X-tremeGENE 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:
• Expression of recombinant proteins for functional analysis.
• Physiological studies of metabolic pathways.
• Analysis of regulatory sequences using reporter gene assays.
• Gene expression assays.
• Cancer research studies.
• Target evaluation.

Features and Benefits

• Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
• Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
• Avoid time-consuming optimization procedures in commonly used cell lines.

Quality

Each lot of X-tremeGENE 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

Legal Information

X-tremeGENE is a trademark of Roche

Safety & Documentation

Safety Information

RIDADR 
UN1170 - class 3 - PG 2 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalia...
Keywords: Antibiotics, Diagnostic, Gene expression, Genetics, Transfection, Western blot

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA enco...
Keywords: Diagnostic, Gene knockdown, Transcription, Transfection

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques serve as an analytical too...
Keywords: Apoptosis, Biological techniques, Drug discovery, Gene expression, Genetic, Precipitation, Transduction, Transfection, transformation

Molecular Biology Handbook

Molecular Biology is the science that aims to understand biological activity at the molecular level. These biological activities usually involve the plant or animal cell, and the nucleic acids and pr...
Keywords: Cell culture, Cell signaling, Cloning, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, Molecular biology, Molecular biology techniques, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction - quantitative, Protein electrophoresis, Purification, Transfection, Western blot, transformation

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Reverse transfection of plasmid DNA

Automation is used for many applications to reduce variation caused by manual han­dling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown s...
Keywords: Cell culture, Transfection

Roche Transfection Database

X-tremeGENE™ Transfection Reagents from Roche have been used for a wide range of applications and a vast array of cell lines, primary cells and stem cells. Use the table below to find the right produ...
Keywords: Apoptosis, Cancer, Clinical, Cloning, DNA replication, Gene expression, Immunoprecipitation, In Situ hybridization, Individual protein Immunoprecipitation, Ion-exchange chromatography, Methylations, Microscopy, Nucleic acid hybridization, Polymerase chain reaction, Recombination, Transcription, Transfection, Western blot

Transfection Best Practices

Successful transfection of nucleic acids and proteins depends on numerous factors that range from reagent and cell type selection to protocol optimization. Ensure optimal quality in your experiment b...
Keywords: Antibiotics, Apoptosis, Growth factors, Molecular biology, Purification, Transfection

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
Keywords: Infrared spectroscopy, Ion-exchange chromatography, Transfection

X-tremeGENE™ Transfection Reagents General Recommendations

The ability to successfully transfect a cell varies significantly depending on the cell type, protocol, and the composition of the transfection reagent. X-tremeGENE™ transfection reagents are designe...
Keywords: Cancer, Cell culture, Cellular processes, Diagnostic, Gene expression, Gene knockdown, Microscopy, Transfection

Protocols

Protocols for Transfecting the Most Common Cell Lines with X-tremeGENE™ Transfection Reagents

X-tremeGENE™ transfection reagents provide reliable transfection of diverse nucleic acids and CRISPR/Cas9 components, a critical step for your molecular and cellular research. Explore our list of the...
Keywords: Diagnostic, Gene expression, Transfection

X-tremeGENE HP DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
Keywords: Gene expression, Transfection

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70–90 % confluency).
Keywords: Gene expression, Transfection

Related Content

How Transfection Works [VIDEO]

Transfection is the process that introduces DNA or RNA into eukaryotic cells. It is a common and essential method to study and modulate gene regulation, which allows for a better understanding of gen...
Keywords: Gene expression, Genomics, Proteomics, Transfection

Transfection Efficiency and Optimization

The two main purposes of transfection are to produce recombinant proteins and to specifically enhance or inhibit gene expression in transfected cells. As such, transfection is a powerful analytical t...
Keywords: Gene expression, Transfection

Peer-Reviewed Papers
15

References

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NA0300 GenElute HP Plasmid Maxiprep Kit, sufficient for 10 purifications
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PP2393 Mammalian FLAG® Tag Vector Set, plasmid vectors for molecular cloning

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