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33247 Sigma-Aldrich

Urea

puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 57648752

  •  NACRES NA.21

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Properties

Related Categories Analytical Reagents, Analytical Reagents for General Use, Analytical/Chromatography, Puriss p.a. ACS, T-Z, Puriss p.a. ACS More...
Quality Level   200
grade   ACS reagent, reag. Ph. Eur.
  puriss. p.a.
assay   ≥99%
  99-101% (calc. on dry substance)
form   solid
impurities   alkalic reac. substances, complies
  ≤0.0005% heavy metals (as Pb)
  ≤0.05% ammonium (NH4)
  ≤0.1% biuret
ign. residue   ≤0.01% (as SO4)
loss   ≤1.0% loss on drying, 105°C, 1 h
mp   132-135 °C (lit.)
  132-135 °C
solubility   H2O: soluble 480 g/L at 20 °C
density   1.335 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Fe: ≤2 mg/kg
suitability   in accordance for appearance of solution
  in accordance for identity
SMILES string   NC(N)=O
InChI   1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.

Urea is the most commonly used slow release nitrogen fertilizer.

Application

Urea has been used in the following studies:
• Synthesis of SnO2 nanoparticles.
• Synthesis of urethanes, ureides and semicarbazides.
• Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain.
• Protein electrophoretic studies.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
YR6250000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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