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901767 Sigma-Aldrich

PC biotin-PEG3-NHS ester

Synonym: PC biotin-NHS ester

  • CAS Number 2353409-93-3

  • Empirical Formula (Hill Notation) C36H52N6O15S

  • Molecular Weight 840.89

  •  NACRES NA.22

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Properties

Related Categories Chemical Biology, Chemical Synthesis, Crosslinkers, Linkers and Crosslinkers, Other Reactivities More...
form   solid
reaction suitability   reagent type: cross-linking reagent
mp   170 °C
storage temp.   −20°C

Description

Application

PC biotin-NHS ester is a unique amine-reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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