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902020 Sigma-Aldrich

Desthiobiotin-PEG3-Azide

  • CAS Number 1951424-99-9

  • Empirical Formula (Hill Notation) C18H34N6O5

  • Molecular Weight 414.50

  •  MDL number MFCD28334534

  •  NACRES NA.22

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Properties

form   paste
storage temp.   −20°C

Description

Application

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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