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51456 Sigma-Aldrich

Urea

BioUltra, for molecular biology, ≥99% (T)

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein/REAXYS Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 57651307

  •  NACRES NA.25

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Chaotropic Agents, DNA & RNA Purification,
Quality Level   200
grade   for molecular biology
product line   BioUltra
assay   ≥99% (T)
form   powder or crystals
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.1% biuret
  <0.005% formaldehyde (HCHO)
ign. residue   ≤0.01% (as SO4)
loss   ≤0.1% loss on drying, 20 °C (HV)
pH   8.0-10.0 (20 °C, 8 M in H2O)
mp   132-135 °C (lit.)
solubility   H2O: 8 M at 20 °C, clear, colorless
density   1.335 g/mL at 25 °C (lit.)
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
SMILES string   NC(N)=O
λ   8 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.03
  λ: 280 nm Amax: ≤0.02
InChI   1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Other Notes

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
YR6250000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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