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CRISPR Sigma-Aldrich

MISSION® CRISPR/Cas9 Products and Services

Design and order CRISPR gRNA, Cas9, screening libraries, controls and companion products. Formats include plant, lentivirus, IVT-RNA, plasmid, synthetic, and protein.

Synonym: CRISPR, CRISPRs, Cas9, Crispr RNA, Crispr/Cas System, Synthetic CRISPR, crRNA, tracrRNA

Guide RNAs

Visit CRISPR Essentials to review CRISPR formats and products that best fit your research needs.

Order Guaranteed Predesigned CRISPR gRNAs

  • Synthetic SygRNA® gRNAs available in two-part (crRNA:tracrRNA) or one-part sgRNA formats. 
  • Perfect for animal model generation and cell model engineering.
  • Recommended to complex with Cas9 protein.
  • Lentivirus gRNAs are available as ready-to-use lentivirus transduction particles. 
  • Perfect for difficult-to-transfect or primary cells.
  • Plasmid DNA transfection-grade plasmid gRNAs available in a variety of vectors.
  • Perfect for transfection, nucleofection, and lentiviral packaging (Note: lentiviral packaging can only be achieved by selecting a lentiviral vector).
  • In Vitro Transcribed RNA  is provided in DNase-, RNase-, and protease-free molecular-grade water.


  • Click on the CRISPR Design Tool to get started.
  • Select your desired genome, choose your gene of interest, and set parameters for the designs.
  • View and export a list of ranked gRNAs.

Order Your CRISPR  gRNAs

*We guarantee the performance of all predesigned Mission™ SygRNA® CRISPR. If your Mission™ predesigned synthetic guide RNAs do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.
To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our available positive controls, side-by-side with the negative results from your predesigned Mission™ SygRNA® CRISPR. To receive your replacement, simply email CRISPR@sial.com and include sample data from a representative experiment (T7E1, TIDE, or NGS), specifying the Mission™ gRNA ID used.

Cas9 Products

  • Proteins — Choose from the largest portfolio of Cas9 proteins available for any application.
  • Plasmid DNA — Cas9 expression plasmids utilize the CMV promoter for effective, transient expression. Available with and without fluorophore and antibiotic selection elements.
  • Lentivirus — Select Cas9-expressing lentivirus transduction particles, with blasticidin or neomycin resistance.
  • CRISPR Activators — Synergistic activation mediators (SAM) are potent transcriptional activation protein complexes. Our SAM CRISPRa system uses CRISPR-Cas9-mediated guidance to target SAM components to gene promoters, enabling site-specific transcriptional activation of a gene of interest. 


CRISPR Screening Tools: Lentiviral Libraries, Collections, and Panels

Sanger Whole Genome Knockout (KO) Arrayed CRISPR Libraries — R&D 100 Award Winner (2016)

Your solution for screening the human and mouse whole genomes for drug discovery or target identification.

  • Over 74,000 individual off-the-shelf CRISPR gRNAs designed to maximize KO of every protein-coding gene in human and mouse genomes.
  • Available as individual clones, gene panels, and whole genome libraries.
  • Learn more about Sanger Arrayed CRISPR Libraries.

Sanger QuickPick™ KO gRNAs

Order off-the-shelf KO gRNAs from the award-winning Sanger Whole Genome KO Arrayed CRISPR Libraries.

Whole Genome KO Pooled Libraries

Whole Genome Activator Libraries

Synergistic Activation Mediators (SAM) are potent transcriptional activation protein complexes.The MISSION™ SAM CRISPRa system uses CRISPR-Cas9-mediated guidance to target SAM components to gene promoters, enabling site-specific transcriptional activation of a gene of interest.

Deconvolution Services

Controls and Companion Products 

  • Controls — Positive and negative controls in plasmid and lentiviral formats, available in a variety of vector options.
  • TracrRNA — Chemically modified and unmodified versions required to pair with crRNA.
  • Transfection Reagents — Transfection/delivery products for use with Sigma-Aldrich® plasmid DNA, Synthetic RNA, and Cas9 Ribonucleoprotein (RNP) Complexes.
  • Lentiviral Packaging Mix — Recommended for packaging Lenti-CRISPR plasmid DNA into pseudo-typed lentiviral particles for downstream transduction applications. 
  • Ready-to-ship in two business days.
  • CRISPR Integration Kit — Includes a validated CRISPR that cleaves DNA with high efficiency and a  standardized oligo donor designed to survey homology dependent repair frequencies in a wide variety of cell lines.

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?