A CRISPR/Cas-GFP Vector for Rapid Expression Verification and Enrichment of Genome Edited Cells
In many genome editing experiments involving ZFNs and CRISPR/Cas nucleases, the first challenge is achieving successful delivery of plasmids and subsequent expression of the encoded nucleases. While ...
Keywords: Cloning, Gene expression, Sequencing, Transfection
CRISPR Use License Agreement
This Product and its use are covered under a license between The Broad Institute, Inc. (BROAD) and Sigma-Aldrich Co. LLC (SIGMA) and are the subject of one or more of the following patents controlled...
Keywords: Agriculture, Clinical, Diagnostic, Substitutions, Veterinary
CRISPR on the Brain? Standardized Functional Genomics Tools Advance Neuroscience
Deciphering the genetics of neuroscience has always been challenging. Heterogeneous tissue microenvironments, complex genetic interactions and phenotypes, lack of model systems that accurately mimic ...
Keywords: Functional genomics, Gene expression, Genetic, Genetics, Genomics, Neuroscience, Sequencing
CRISPR/Cas Nuclease RNA-guided Genome Editing
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Keywords: Acetylations, Catalysis, Cell culture, Degradations, Gene expression, Genetic, PAGE, Polymorphisms, Transcription, Transduction, Transfection
Creating Transgenic Mice using CRISPR-Cas9 Genome Editing
While several genome editing tools have been developed in recent years, including zinc finger-based strategies and TALENs (transcription activator-like effector nucleases), none have been as efficien...
Keywords: Amplification, Cell culture, Enzyme-linked immunosorbent assay, Gene expression, Genetic, Genetics, Immunohistochemistry, Immunology, Nutrition, Polymerase chain reaction, Recombination, Southern blot, Transcription, Transfection, Western blot
Safe Handling of Lentivirus
Our lentiviral vector systems have been developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication, including eliminating viru...
Keywords: Environmental, Genetic, Recombination, Transfection
Tips for Cell Engineering using Cas9-GFP CRISPR plasmids
CRISPR endonucleases have shown wide variation in their activity, even among multiple CRISPRs designed within close genomic proximity.1 For this reason, we highly recommend that you test 3 to 4 CRIS...
Keywords: Cell culture, Cloning, DNA purification, Gene expression, Microscopy, Purification, Reductions, Sequencing, Transcription, Transfection
Application Note: Synthetic CRISPR - Cas9 Ribonucleoprotein (RNP)
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA. In th...
Keywords: Cell culture, Centrifugation, Culture media, Degradations, Nucleic acid annealing, Phase transitions, Polymerase chain reaction, Transfection
CRISPR Lentiviral Screening: Antibiotic Selection
A kill curve is a dose-response experiment where cells are subjected to a range of antibiotic concentrations to determine the minimum concentration of antibiotic needed to kill all cells over a desir...
Keywords: Antibiotics, Apoptosis, Gene expression, Transduction
Genome engineering using the CRISPR-Cas9 system
F Ann Ran a, b, c, d, e , Patrick D Hsu a, b, c, d, e , Jason Wright a , Vineeta Agarwala a, f, g , David A Scott a, b, c, d , Feng Zhang a, b, c, d
Keywords: Amplification, Antibiotics, Antivirals, Apoptosis, Catalysis, Cell culture, Cell dissociation, Central Nervous System, Chromatin immunoprecipitation, Cloning, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Genetics, Growth factors, Melting, Nucleic acid annealing, PAGE, Peptide synthesis, Polymerase chain reaction, Polymorphisms, Purification, Rearrangements, Recombination, Sample preparations, Sequencing, Transcription, Transfection, transformation
Protocol, FACS Titration of Lentivirus
FACS Analysis of CFU FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scat...
Keywords: Enzyme-linked immunosorbent assay, Flow cytometry, Gene expression, Titrations, Transduction
Successful Transduction Using Lentivirus
Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts. As...
Keywords: Antibiotics, Enzyme-linked immunosorbent assay, Gene expression, PAGE, Polymerase chain reaction, Titrations, Transduction
CRISPR Webinars [VIDEOS]
CRISPR-Cas9 nucleases have revolutionized genome editing enabling unprecedented efficiency of targeted mutagenesis. The ability to perform large-scale, whole genome loss-of-function screens has allow...
Keywords: Antibiotics, Cell culture, Cloning, Functional genomics, Gene expression, Genetic, Genomics, Molecular biology, Polymorphisms, Sequencing, Transfection
In recent years CRISPR has revolutionized gene editing capabilities, leading to sophisticated ways to create success with any experiment. As the first company to offer custom biomolecules globally fo...
Keywords: Genetic, Transduction, Transfection
Frequently Asked Questions: SygRNA Synthetic CRISPR
For easy-to-transfect cell lines, we suggest unmodified/desalted. For difficult-to-transfect cell lines such as primary cells, we recommend modified/HPLC-purified. Most animal model researchers prefe...
Keywords: Chromatin immunoprecipitation, Degradations, High performance liquid chromatography, Nucleic acid annealing, Polymorphisms, Sequencing, Transcription, Transduction, Transfection, Western blot
Predictive Models for Neuroscience using CRISPR [VIDEO]
Caroline Beckett, the global CRISPR product manager for MilliporeSigma, discusses reagent solutions for creating predictive models for neuroscience research. She notes that many neuroscientists want ...
Keywords: Cell culture, Diseases, Neurodegenerative Diseases, Neuroscience
Synthetic Biology Resources
We are using our stregnths to help build the field of Synthetic Biology. Below is a collection of links to our favorite resources. In addtion to these resources, we also offer thousands of products f...
Keywords: Cell culture, Cloning, Gene expression, Genetic, Genetics, Metabolic Pathways, Metabolites, Molecular biology, Molecular biology techniques, Photosynthesis, Poisons, Polymerase chain reaction - quantitative, Transcription, Transfection