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  • P8609 - Anti-Serine/Threonine Protein Phosphatase 2C α/β antibody produced in rabbit

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P8609 Sigma-Aldrich

Anti-Serine/Threonine Protein Phosphatase 2C α/β antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
species reactivity   mouse, bovine, rabbit, human
application(s)   immunoprecipitation (IP): suitable
  microarray: suitable
  western blot: 1:500-1:1,000 using total rat brain homogenate
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen 44-46 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PPM1A(5494), PPM1B(5495)
mouse ... Ppp2ca(19052), Ppp2cb(19053)
Quality Level   200
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated
UniProt accession no.   P35813

Description

Immunogen

synthetic peptide corresponding to amino acids 23-37 of the protein phosphatase 2C α/β structural subunit.

General description

Among the post-translational modifications, phosphorylation is a vital regulatory mechanism of key proteins involved in specific pathways. Reverse phosphorylation has become recognized as the key process of regulation of gene expression, cellular proliferation, differentiation in Eukaryotes. Protein phosphatases, like kinases, are a class of enzymes that regulate protein phosphorylation. The serine/threonine phosphatases have been classified into four groups which include PP1, PP2A, PP2B (also termed calcineurin) and PP2C on the basis of differences in their biochemical properties. Protein phosphatases 2C (PP2Cs) are serine/threonine protein phosphatases primarily involved in stress responses. Two isoforms PP2C α and PP2Cβ are reported. Functionally, PP2C isoforms suppress TGF-β-activated kinase (TAK1), STAT3 and the nuclear factor κB pathway.
Anti-Serine/Threonine Protein Phosphatase 2C α/β specifically recognizes 44-46 kDa protein phosphatase 2C α?and β?isoforms.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide

Application

The recommended working dilution is 1:1000 to 1:5000 for immunoblotting using peroxidase conjugated goat anti-rabbit IgG and chemiluminescent detection. The recommended working dilution for immunoprecipitation is 1:1000 to 1:5000. The antibody is suitable for protein microarray.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Flash Point(F)  Not applicable
Flash Point(C)  Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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