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R4775 Sigma-Aldrich

REDExtract-N-Amp PCR ReadyMix

Ready-to-use 2X PCR Master Mix with Loading Dye

  •  NACRES NA.55

Purchase

Properties

Related Categories Cell Biology, DNA & RNA Purification, General Stem Cell Biology, Genomic DNA Amplification, Genomic DNA Purification,
Quality Level   200
usage   1.2 mL sufficient for 100 amplifications
  12 mL sufficient for 1000 amplifications
  125 mL sufficient for 10000 amplifications
feature   hotstart
color   red
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

General description

The REDExtract-N-Amp PCR reaction mix is a PCR Master mix containing buffer, salts, dNTPs, and REDTaq DNA polymerase.

PCR ReadyMix is intended for use with Sigma′s Extract-N-Amp Plant PCR kit and Extract-N-Amp Tissue PCR Kit. All Extract-N-Amp kits include a PCR ReadyMix sufficient for one PCR reaction per extraction. However, where additional PCR reactions are required, supplemental PCR ReadyMix may be needed. The REDExtract-N-Amp PCR ReadyMix contains a inert tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp PCR Master Mix has been used as a component of PCR reaction for the genotyping of fixed samples. It has been used in PCR amplification as a component of the PCR reaction mix containing the DNA isolated from lichen family using the REDExtract-N-Amp Plant PCR Kit. It has also been used in the PCR reaction mix for the amplification of DNA isolated from single yeast colonies and fungal spores using REDExtract-N-Amp seed PCR kit.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Extract-N-Amp™ Tissue Feature Article By: Scott Weber and Derek Douglas

Introduction Materials and Methods Results and Discussion Conclusions Acknowledgements References Ordering Information
Scott Weber and Derek Douglas
St. Louis, MO, USA
Keywords: Amplification, Digestions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Sequencing

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Solving the Space Constraints of High-Throughput Genotyping

High-throughput genotyping is a critical tool for modern plant breeding programs. The more samples analyzed, the more marker data collected, and the better the chances to identify traits that enhance...
Keywords: Agriculture, Sample preparations

Protocols

REDExtract-N-Amp™ Plant PCR Kit Protocol

The REDExtract-N-Amp Plant PCR Kits contain all the reagents needed to rapidly extract and amplify genomic DNA from plant leaves. Briefly, the DNA is extracted from a piece of leaf tissue, a 0.5 to 0...
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing

REDExtract-N-Amp™ Seed PCR Protocol - Technical Document

The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from seeds (soybean, corn, wheat, etc.). Briefly, DNA is extracted from ground seed mater...
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Precipitation, Purification, Sequencing

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. Brief...
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Precipitation, Purification, Sequencing

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
Keywords: Building blocks, Mass spectrometry, Organic synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Vitamins

Peer-Reviewed Papers
15

References

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XNAP REDExtract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
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XNAPE REDExtract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications
XNAP2 Extract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
XNAR Extract-N-Amp Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAPR REDExtract-N-Amp Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAPS REDExtract-N-Amp Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications
XNAT2 Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
XNAT2R Extract-N-Amp Tissue PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAT REDExtract-N-Amp Tissue PCR Kit
E3004 Extract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
E8155 Extract-N-Amp PCR Diluent
E7526 Extraction Solution, Molecular Biology
D5688 Dilution Solution, Extract-N-Amp Plant Dilution Solution
XNAPG SYBR® Green Extract-N-Amp Plant PCR Kit, sufficient for 100 preparations
S4320 SYBR® Green Extract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
XNATG SYBR® Green Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
T3073 Tissue Preparation Solution, 30 mL sufficient for 1,000 extractions
E0781 Extract-N-Amp PCR ReadyMix with qPCR Reference dye
I9516 2-Propanol, BioReagent, for molecular biology, ≥99.5%
D8418 Dimethyl sulfoxide, for molecular biology
B7906 1-Butanol, for molecular biology, ≥99%
M5904 Mineral oil, for molecular biology, BioReagent, light oil
C0549 Chloroform:Isoamyl alcohol 24:1, suitable for nucleic acid purification
B0300 Betaine solution, 5 M, PCR Reagent
W4502 Water, Nuclease-Free Water, for Molecular Biology

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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