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S4320 Sigma-Aldrich

SYBR® Green Extract-N-Amp PCR ReadyMix

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

  •  NACRES NA.31

Purchase

Properties

Related Categories Biochemicals and Reagents, Fluorescent Kits, Fluorescent Probes, Labels, Particles and Stains More...
Quality Level   200
usage   1.2 mL sufficient for 100 amplifications
  12 mL sufficient for 1000 amplifications
  125 mL sufficient for 10000 amplifications
shipped in   wet ice
storage temp.   −20°C

Description

General description

The SYBR Green Extract-N-Amp PCR ReadyMix is the specially formulated PCR enzyme blend contained in the SYBR Green Extract-N-Amp Plant PCR kit and SYBR Green Extract-N-Amp Tissue PCR Kit. It is intended to supplement these kits for researchers who perform more than one amplification per extracted sample.

Packaging

1.2 mL is sufficient for 100 amplifications as described in kit protocols (20 μl total reaction volume)

12 mL is sufficient for 1000 amplifications as described in kit protocols (20 μl total reaction volume)

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Solving the Space Constraints of High-Throughput Genotyping

High-throughput genotyping is a critical tool for modern plant breeding programs. The more samples analyzed, the more marker data collected, and the better the chances to identify traits that enhance...
Keywords: Agriculture, Sample preparations

Protocols

SYBR® Green Extract-N-Amp™ Plant PCR Kit Protocol

The SYBR Green Extract-N-Amp Plant PCR Kit contains the reagents needed for rapid extraction, amplification and detection of genomic DNA from plant leaves. DNA is rapidly extracted from a piece of le...
Keywords: Amplification, Degradations, Diagnostic, Gas chromatography, Metabolites, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Size-exclusion chromatography

SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol

Product Description Reagents and equipment required but not provided Precautions and Disclaimer Storage of Kit Components Preliminary Considerations Procedure Troubleshooting Guide Materials Referenc...
Keywords: Amplification, Degradations, Diagnostic, Gas chromatography, Metabolites, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Phase transitions, Polymerase chain reaction, Precipitation, Purification

Peer-Reviewed Papers
15

References

Related Products

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Add to Cart

XNAP REDExtract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
XNAPE REDExtract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications
XNAP2 Extract-N-Amp Plant PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
XNAR Extract-N-Amp Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAPR REDExtract-N-Amp Plant PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAPS REDExtract-N-Amp Plant PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications
XNAT2 Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
XNAT2R Extract-N-Amp Tissue PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAT REDExtract-N-Amp Tissue PCR Kit
R4775 REDExtract-N-Amp PCR ReadyMix, Ready-to-use 2X PCR Master Mix with Loading Dye
E3004 Extract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
E8155 Extract-N-Amp PCR Diluent
E7526 Extraction Solution, Molecular Biology
D5688 Dilution Solution, Extract-N-Amp Plant Dilution Solution
XNAPG SYBR® Green Extract-N-Amp Plant PCR Kit, sufficient for 100 preparations
XNATG SYBR® Green Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
T3073 Tissue Preparation Solution, 30 mL sufficient for 1,000 extractions
E0781 Extract-N-Amp PCR ReadyMix with qPCR Reference dye
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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