Articles
Improving Real-Time PCR Success
To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction
Metagenomics and Quantitative PCR
The highly variable regions within the 16S ribosomal RNA (rRNA) gene sequences are being exploited with PCR and metagenomic sequencing to characterize the microbial strains within the gastrointestina...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Gastrointestinal, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Probiotics, Sequencing
Polymerase Chain Reaction | PCR Process & Guide
Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Amplification, Cloning, Degradations, Detection methods, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Indicators, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Size-exclusion chromatography, Transcription
Real-time PCR Study Report on Nancy-520
Sigma-Aldrich offers a fluorescent stain for dsDNA called Nancy-520, a greener alternative to Ethidium bromide. This dye has also been successful in PCR (Real time-PCR) applications. In order to deve...
Keywords: Amplification, Centrifugation, Electrophoresis, Gel electrophoresis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Titrations
Transplex Whole Transcriptome RNA Amplifications
The efficacy of amplification of small quantities of total RNA with the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study. Total RNA extracted from decreasin...
Ken Heuermann, Brian Ward
Biowire Volume 10 Article 1
Keywords: AGE, Amplification, Applications, Cancer, Clinical, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genomics, Instructions, Marketing, Methods, Microarray Analysis, Microbiology, Nucleic acid annealing, Nucleic acid hybridization, Oligonucleotide microarrays, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Reductions, Transcription
Using probe-based quantitative PCR to measure gene-level expression
Quantitative PCR has reached a level of sensitivity, accuracy, and ease to support use as a routine assay for measuring gene-level expression. The field of cancer research is validating a number of a...
BioFiles Vol. 4, No. 7
Keywords: Amplification, Cancer, Clinical, Diagnostic, Gene expression, Genetic, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Sample preparations, Size-exclusion chromatography
Hot Start Amplification of DNA with SYBR® Green JumpStart™ Taq ReadyMix™
The single most important step in assuring success with PCR is high quality DNA preparation. Integrity and purity of DNA template is essential. Quantitative PCR involves multiple rounds of enzymatic ...
Keywords: AGE, Amplification, Diagnostic, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Separation, Size-exclusion chromatography, Solvents
SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms. Microgram quantit...
Keywords: Amplification, Centrifugation, Condensations, Degradations, Electrophoresis, Eliminations, Enzyme activity, Gene expression, Genomics, Microarray Analysis, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Sample preparations, Sequencing, Sonication
Universal SYBR Green qPCR Protocol
Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Melting, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Size-exclusion chromatography, Solvents, Titrations
Agriculture | Genotyping
Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification
Automated Protocols for Life Science Research
Our scientists have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply information such as i...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative
Coronavirus COVID-19 (SARS-CoV-2) Detection, Characterization, Vaccine and Therapy Production
As a global life science supplier, we are here to help in the response to COVID-19. From supplying raw materials and research products for detection and characterization to production, we’ve consolid...
Keywords: PAGE
PCR Selection Guide
We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative
Plant Breeding Workflow - Collaborating to Empower Yield Improvements
Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
Keywords: Building blocks, Mass spectrometry, Organic synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Vitamins