S4438 Sigma-Aldrich

SYBR^® Green JumpStart^™ Taq ReadyMix^™

for quantitative PCR, MgCI₂ in buffer

MDL number MFCD03458666
NACRES NA.55

SYBR® Green JumpStart™ Taq ReadyMix™ for quantitative PCR, MgCI2 in buffer

SDS

Properties

Related Categories	Biochemicals and Reagents, Core Bioreagents, Fluorescent Kits, Fluorescent Probes, Labels, Particles and Stains, Life Science Reagents for PCR, Molecular Biology, Nucleic Acid Amplification, PCR Enzymes, PCR/Amplification, Plant Biotechnology, Plant Molecular Biology, Quantitative PCR, Research Essentials, SYBR Green Based Quantitative PCR, SYBR Green Quantitative PCR, Traditional QPCR More...
Quality Level	200
form	liquid
feature	hotstart
concentration	1.25 units/reaction (50 μL reaction volume)
Featured Industry	Agriculture
compatibility	for use with ABI 5700
shipped in	wet ice
storage temp.	−20°C

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Description

General description

SYBR^® Green I is ideal for quantifying any DNA sequence. The dye binds to double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout cycling.

Other Notes

SYBR Green JumpStart Taq ReadyMix combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate for ease of use. Simply add 25 μL of the 2× mix to DNA template, primers and water. The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate C_T values.

Features and Benefits

• Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required.
• JumpStart Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield.
• SYBR Green JumpStart Taq ReadyMix for SYBR based QPCR is formulated with MgCl₂ or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments.

• This master mix allows consistency from one reaction to the next
• Designed to minimize contamination
• Reduced primer dimers
• Reduced set-up time as compared to manual or wax HotStart^® methods
• Allows for room temperature set-up and contains a fluorescent dye; Ideal for qPCR applications

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL

Principle

SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments and can also be used for PCR optimization prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Documents

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SDS

Genotyping Workflow (2 MB)

Novel Whole Genome Amplification Poster (3 MB )

PCR Prod Guide (234 KB )

PCR Tips (4 KB )

S4438 - Bulletin (163KB)

Single Cell WGA Poster (546 KB )

Technical Article Jan02 (193 KB )

Technical Article Jan03 (223 KB )

Protocols & Articles

Articles

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Metagenomics and Quantitative PCR

The highly variable regions within the 16S ribosomal RNA (rRNA) gene sequences are being exploited with PCR and metagenomic sequencing to characterize the microbial strains within the gastrointestina...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Gastrointestinal, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Probiotics, Sequencing

Polymerase Chain Reaction | PCR Process & Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Amplification, Cloning, Degradations, Detection methods, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Indicators, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Size-exclusion chromatography, Transcription

Real-time PCR Study Report on Nancy-520

Sigma-Aldrich offers a fluorescent stain for dsDNA called Nancy-520, a greener alternative to Ethidium bromide. This dye has also been successful in PCR (Real time-PCR) applications. In order to deve...
Keywords: Amplification, Centrifugation, Electrophoresis, Gel electrophoresis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Titrations

Transplex Whole Transcriptome RNA Amplifications

The efficacy of amplification of small quantities of total RNA with the TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study. Total RNA extracted from decreasin...
Ken Heuermann, Brian Ward
Biowire Volume 10 Article 1
Keywords: AGE, Amplification, Applications, Cancer, Clinical, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genomics, Instructions, Marketing, Methods, Microarray Analysis, Microbiology, Nucleic acid annealing, Nucleic acid hybridization, Oligonucleotide microarrays, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Reductions, Transcription

Using probe-based quantitative PCR to measure gene-level expression

Quantitative PCR has reached a level of sensitivity, accuracy, and ease to support use as a routine assay for measuring gene-level expression. The field of cancer research is validating a number of a...
BioFiles Vol. 4, No. 7
Keywords: Amplification, Cancer, Clinical, Diagnostic, Gene expression, Genetic, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Sample preparations, Size-exclusion chromatography

Protocols

Hot Start Amplification of DNA with SYBR® Green JumpStart™ Taq ReadyMix™

The single most important step in assuring success with PCR is high quality DNA preparation. Integrity and purity of DNA template is essential. Quantitative PCR involves multiple rounds of enzymatic ...
Keywords: AGE, Amplification, Diagnostic, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Separation, Size-exclusion chromatography, Solvents

SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms. Microgram quantit...
Keywords: Amplification, Centrifugation, Condensations, Degradations, Electrophoresis, Eliminations, Enzyme activity, Gene expression, Genomics, Microarray Analysis, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Peptide synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymerization reactions, Purification, Sample preparations, Sequencing, Sonication

Universal SYBR Green qPCR Protocol

Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Melting, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Size-exclusion chromatography, Solvents, Titrations

References

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SYBR® Green JumpStart™ Taq ReadyMix™

for quantitative PCR, MgCI2 in buffer