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808541

TSKgel® G3000SWXL HPLC Column

phase diol, L × I.D. 30 cm × 7.8 mm, 5 μm particle size

Synonym: TSKgel® SWXL Columns

  •  eCl@ss 32110501

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Properties

Related Categories Analytical/Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, TSKgel Gel Filtration Columns for Proteins and other Biopolymers, TSKgel Gel Filtration Columns to Analyze mAb Purity and Aggregates,
mfr. no.   Tosoh, 08541
parameter   ≤0.5 M salt concentration
  0-100% aqueous soluble organic solvents
  1.2 mL/min flow rate
  10-30 °C temperature
  72 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   30 cm × 7.8 mm
matrix   silica particle platform
matrix active group   diol phase
particle size   5 μm
pore size   250 Å
operating pH   2.5 - 7.5
suitability   suitable for L20 per USP
  suitable for L59 per USP
separation technique   size exclusion (SEC)

Description

General description

Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution. With 5μm particles and 250Å pores, TSKgel G3000SWXL is an excellent choice for protein and large peptide separations. The fractionation range is applicable for globular protein samples up to 0.5 million Da.

Physical form

Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

2 Micron SEC Column with Dual Functionality for Use in UHPLC and HPLC Systems

Size Exclusion Chromatography (SEC) is an important method for monitoring the impurities of biotherapeutic drugs, in order to minimize the immunologic effect that may occur when such impurities are p...
Phu Duong
Reporter US Volume 34.2
Keywords: Chromatography, High performance liquid chromatography, Separation, Size-exclusion chromatography

Calibration Curves for TSKgel® SW mAb Columns

From our library of Articles, Sigma-Aldrich presents Calibration Curves for TSKgel® SW mAb Columns
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Glutathione S-Transferase on TSKgel® G3000SWXL

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Glutathione S-Transferase on TSKgel® G3000SWXL
Keywords: Chromatography, Enzyme activity, High performance liquid chromatography

HPLC Analysis of Peroxidase on TSKgel® G3000SWXL

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peroxidase on TSKgel® G3000SWXL
Keywords: Chromatography, High performance liquid chromatography

Physical Stability of a Gel Filtration Column for Antibody Analysis

Independent of the mode of liquid chromatography, the lifetime of an HPLC or UHPLC column depends on the characteristics of the packing material, how that material was packed into the column and how ...
Roy Eksteen
Reporter US Volume 31.2
Keywords: Chromatography, Filtration, High performance liquid chromatography, Indicators, Ion Exchange, Ligands, Size-exclusion chromatography, Solvents

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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

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