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144 matches found for SPE

LC/MS Analysis of 3-Methoxytyramine and Metanephrines in Human Plasma on Ascentis® Express Biphenyl after On-line SPE using Supel™Genie RP-Amide Cartridge

SPE tube/cartridge Supel™ Genie On-line SPE Cartridge Starter Kit: RP-Amide 2 cm x 4.0 mm I.D. (55516-U) column Ascentis® Express Biphenyl 10 cm x 2.1 mm I.D., 2.7 μm particles (64065-U) column temp. 25 °C mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in methanol gradient 20 to 10...

HPLC Analysis of Bisphenol A in Water on Ascentis® Express C18 after SPE using Supelclean™ ENVI-18

SPE tube/cartridge Supelclean ENVI-18, 500 mg/6 mL glass SPE tube (54331-U) condition 1 mL 1% formic acid in acetonitrile, then 1 mL water elution 2 mL 1% formic acid in acetonitrile eluate post-treatment 1 mL aliquot of eluent, and evaporate/reconstitute to 0.5 mL in acetonitrile column Ascentis Ex...

LC/MS Analysis of 3-Methoxytyramine and Metanephrines in Synthetic Urine on Ascentis® Express Biphenyl after On-line SPE using Supel™Genie RP-Amide Cartridge

SPE tube/cartridge Supel Genie On-line SPE Cartridge Starter Kit: RP-Amide 2 cm x 4.0 mm I.D. (55516-U) column Asentis Express Biphenyl 10 cm x 2.1 mm I.D., 2.7 μm particles (64065-U) column temp. 25 °C mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in methanol gradient 20 to 100% ...

LC/MS Analysis of 3-Methoxytyramine and Metanephrines in Horse Urine on Ascentis® Express Biphenyl after On-line SPE using Supel™Genie RP-Amide Cartridge

SPE tube/cartridge Supel™ Genie On-line cartridge:Â RP-Amide 2 cm x 4.0 mm I.D. (55516-U) column Ascentis® Express Biphenyl 10 cm x 2.1 mm I.D., 2.7 μm particles (64065-U) column temp. 25 °C mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in methanol gradient 20 to 100% B in 10 min;...

GC Analysis of Sterols (Silylated Derivatives) in Olive Oil (Free Sterol Fraction) on SLB®-5ms after SPE using Discovery DSC-Si

sample preparation SPE (Solid Phase Extraction) sample/matrix olive oil spiked with a mixed sterol standard at 5 µg/g SPE tube/cartridge Discovery DSC-Si, 1 g/6 mL (52656-U) condition 2 x 5 mL hexane sample addition 1 mL elution esters fraction: 2.5 mL of hexane:ethyl acetate (90:10); free sterols f...

GC Analysis of Glycols in Water on SPB®-1000 after SPE using ENVI-Carb™ Plus

sample preparation SPE (Solid Phase Extraction) sample/matrix tap water spiked at 2 µg/mL with glycols SPE tube/cartridge ENVI-Carb Plus reversible cartridge, 400 mg/ 1mL (54812-U) condition 1 mL methylene chloride, 2 x 2 mL methanol, 3 mL deionized water (cartridge not allowed to dry during conditi...

GC Analysis of Phenols in Water on a 5% Phenyl Column after SPE using Supelclean™ ENVI-Chrom P

sample preparation SPE (Solid Phase Extraction) sample/matrix water spiked with phenol compounds SPE tube/cartridge Supelclean ENVI-Chrom P, 250 mg/6 mL (57225-U) condition 6 mL methyl t-butyl or ethyl acetate; 6 mL acetonitrile. Equilibrate with 6 mL DI water sample addition 100 mL water sample. dr...

GC Analysis of FAMEs in Cookies on SLB®-IL111 after SPE Fractionation using Discovery® Ag-Ion

sample preparation SPE (Solid Phase Extraction) sample/matrix 1 g of commercially purchased cookies was ground and subjected to acid digestion and alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 SPE tube/cartridge Discovery Ag-Ion SPE tubes, 750 mg/6 mL (54225...

LC/MS (TOF) Analysis of 2C Amines Drugs of Abuse in Urine on Ascentis® Express HILIC after Solid Phase Extraction (SPE) using Supel™-Select SCX

sample preparation SPE (Solid Phase Extraction) sample/matrix spiked urine, 1 mL SPE well plate Supel-Select SCX SPE 96-well Plate, 30 mg/well (575664-U) condition 1 mL methanol, then 1 mL 0.1% formic acid in water washing 2 x 1 mL 10% methanol in water, 2 x 1 mL 1% formic acid in methanol elution 2...

HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt

sample preparation SPE (Solid Phase Extraction) sample/matrix 0.5 mL porcine serum spiked with 0.5 µg/mL or 1.0 µg/mL each analyte then diluted with 0.5 mL water SPE tube/cartridge Discovery DSC-18Lt, 500 mg/3 mL (52613-U) condition 2 mL methanol; 2 mL DI water sample addition 1 mL at 0.75 mL/min wa...

LC/MS Analysis of Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2 in Urine after SPE using Supel™-Select SCX

sample preparation SPE (Solid Phase Microextraction) sample/matrix 1 mL of synthetic urine spiked with 25 ng/mL extraction process acidified to 3-4 pH with formic acid SPE well plate Supel-Select SCX SPE 96-well Plate, 30 mg/well (575664-U) condition 1 mL 1% formic acid in acetonitrile, then 1 mL wa...

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Water on a 5% Phenyl Column after SPE using Supelclean™ ENVI-18

sample preparation SPE (Solid Phase Extraction) sample/matrix water spiked with PAHs SPE tube/cartridge Supelclean ENVI-18, 500 mg/6 mL (57064) condition 2 x 6 mL toluene:methanol (10:1); 6 mL methanol; 6 mL DI water sample addition 250 mL water sample at 10 mL/min drying 10 min under vacuum elution...

UHPLC/MS Analysis of NBOMe Designer Drugs in Urine on Ascentis® Express C18 after Solid Phase Extraction (SPE) using Supel™-Select HLB

sample preparation SPE (Solid Phase Extraction) sample/matrix human trine spiked at 100 ng/mL each compound SPE well plate Supel-Select HLB 96-well pate, 30 mg/well (575661-U) condition 1 mL methanol followed by 1 mL water sample addition 1 mL washing 1 mL water followed by 1 mL water:methanol (75:2...

HPLC Analysis of Triazine Herbicides in Grass on SUPELCOSIL™ LC-8-DB after SPE using Supelclean™ LC-SCX

sample preparation SPE (Solid Phase Extraction) sample/matrix fresh grass clippings spiked with herbicides at 2 ppm SPE tube/cartridge Supelclean LC-SCX, 500 mg/3 mL (57018) condition 1 mL methylene chloride sample addition 2 mL grass extract. Wash with 2 x 2 mL acetonitrile. washing Dry packing for...

LC/MS Analysis of Triclosan (Irgasan) in Waste Water on Ascentis® Express C18 after SPE using Supel™-Select HLB

sample preparation SPE (Solid Phase Extraction) sample/matrix waste-water treatment plant effluent SPE tube/cartridge Supel Select HLB 60 mg/ 3 mL (54182-U) condition 3 mL methanol, 3 mL distilled water, 3 mL 0.01 M HCl sample addition 50 mL washing wash using 10 mL distilled water, dry for 5 minute...

LC/MS with Online Solid Phase Extraction (SPE-LC/MS) of Thyroid Hormones in Plasma on Ascentis® Express Phenyl-Hexyl using RP-Amide Traps

sample preparation SPE (Solid Phase Extraction) column Ascentis Express Phenyl-Hexyl, 5 cm x 2.1 mm I.D., 2.7 µm particles (53334-U) column temp. ambient mobile phase [A] water; [B] methanol, both with 0.1% acetic acid gradient 5% B for 3 min; to 70% B in 2 min, hold 3 min pressure 1800 psi sample p...

GC Analysis of Pesticides in Fruits and Vegetables on a 14%Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb™

sample preparation SPE (Solid Phase Extraction) sample/matrix extract from 50 g homogenized fruit or vegetable sample SPE tube/cartridge ENVI-Carb, 500 mg/6 mL (57094) sample addition 0.5 mL concentrated sample extract elution 20 mL acetonitrile:toluene (3:1) eluate post-treatment Concentrate to 2 m...

HPLC Analysis of Cortocisteroids in Urine on Discovery® HS F5 after SPE using Discovery® DSC-CN

sample preparation SPE (Solid Phase Extraction) sample/matrix human urine spiked with corticosteroids at 0.5 and 1.0 µg/mL and diluted in DI water (1:1, v/v) SPE well plate Discovery DSC-CN 96-well SPE, 100 mg/well (575636-U) condition 1 mL methanol; 1 mL DI water. sample addition 1 mL spiked urine ...

HPLC Analysis of Amphetamines in Urine on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and Standard C18

sample preparation SPE (Solid Phase Extraction) sample/matrix human urine spiked amphetamine and methylamphetamine at with 2 mg/mL SPE tube/cartridge Discovery DSC-MCAX, 100 mg/3mL (52783-U) condition DSC-MCAX SPE tube: 1 mL methanol; 1 mL 50 mM ammonium acetate, pH 6.0; C-18 SPE tube: 1 mL methanol...

GC Analysis of Pesticides in Spinach on Equity®-1 after SPE using Supelclean™ ENVI-Carb-II/PSA

sample preparation SPE (Solid Phase Extraction) sample/matrix 10 g of fresh spinach spiked at 0.2 ppm each pesticide SPE tube/cartridge Supelclean ENVI-Carb-II/PSA, 500mg/300mg/6mL (55119-U) condition 5 mL acetonitrile:toluene (3:1, v/v) sample addition 1 mL concentrated sample extract elution 20 mL...

LC/MS/MS of Digoxin and Digitoxin in Serum or Plasma on Titan™ C18 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects

sample preparation SPE (Solid Phase Extraction) sample/matrix rat plasma or human serum spiked at 0.05 ng/mL with digitoxin and digoxin SPE well plate HybridSPE-PLus 96-well Plate, 50 mg/well (575659-U) sample addition 100 µL spiked rat plasma followed by 300 µL 1% formic acid in acetonitrile elutio...

LC/MS (TOF) Analysis of Metanephrine and Normetanephrine in Urine on Ascentis® Express OH5 after Sold Phase Extraction (SPE) using Supel™-Select SCX

sample preparation SPE (Solid Phase Extraction) sample/matrix human urine spiked at 20 ng/mL each analyte SPE well plate Supel-Select SCX 96-well plate, 30 mg/well (575664-U) condition 1 mL 1% formic acid in acetonitrile, 1 mL water sample addition 1 mL spiked urine washing 2 mL water, 1 mL methanol...

LC/MS (TOF) Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC: Improvement in Analyte Response after Solid Phase Extraction (SPE) using HybridSPE®-Phospholipid

sample preparation SPE (Solid Phaes Extraction) SPE tube/cartridge HybridSPE-Phospholipid 96-Well Plate, 50 mg/2 mL (575656-U) sample addition apply 100 µL of plasma to plate, followed by 300 µL of 1% formic acid acetonitrile. Agitate via vortex for 4 min elution place on vacuum manifold and apply 1...

HPLC Analysis of Dermorphin in Horse Plasma on Ascentis® Express F5 after SPE with HybridSPE®

sample preparation SPE (Solid Phase Extraction) sample/matrix horse plasma spiked at 10 µg/mL SPE tube/cartridge [TM"HybridSPE"]-Phospholipid Plate, 50mg/96-Well (575656-U) sample addition 3:1, (1% formic acid in acetonitrile); plasma column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles...

UHPLC/MS/MS Analysis of Benzodiazepines and Glucuronide Metabolites in Urine on Titan™ C18 after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion

sample preparation SPE (Solid Phase Extraction) SPE well plate Supel-Select HLB 96-well plate, 60 mg/well (575662-U) condition 1 mL acetonitrile, 1 mL water sample addition 1.1 mL enzyme-digested urine washing 1 mL water, 1 mL 25% methanol elution 1 mL acetonitrile column Titan C18, 5 cm x 2.1 mm I....

GC Analysis of Organophosphorus Pesticides (OP-Pest) in Cabbage, Onion, and Mushrooms on SLB-5ms after SPE using Supelclean™ ENVI-Carb™ II/PSA

sample preparation SPE (Solid Phase Extraction) sample/matrix 10 g homogenized food sample (green onions, apples, Napa cabbage, button mushrooms) SPE tube/cartridge Supelclean ENVI-Carb II/PSA SPE Tube (54067-U) condition 5 mL acetone:toluene (65:35) sample addition 1 mL sample extract elution 10 mL...

HPLC Analysis of Diazepam and Metabolites in Serum on Discovery® C18 after SPE using Discovery® DSC-8

sample preparation SPE (Solid Phase Extraction) sample/matrix goat serum spiked with diazepam and metabolites at 0.5 µg/mL and diluted 1:1 in 25 mM ammonium formate, pH 7.1 SPE well plate Discovery DSC-8 96-well SPE Plate, 100 mg/well (575627-U) condition 1 mL methanol followed by 1 mL DI water. sam...

LC/MS Analysis of Amphetamine Enantiomers on Astec CHIROBIOTIC® V2 in Urine after Solid Phase Extraction (SPE) using Supel™-Select SCX

sample preparation SPE (Solid Phase Extraction) sample/matrix 1 mL of synthetic urine spiked with 50 ng/mL of each enantiomer SPE well plate Supel-Select SCX, 96-well plate, 30 mg/well (575664-U) condition 1 mL 1% formic acid in acetonitrile followed by 1 mL water sample addition 1 mL spiked urine s...

LC/MS/MS Analysis of Immunosuppressant Drugs in Human Plasma on Ascentis® Express F5 after SPE Using HybridSPE®-Phospholipid

sample preparation SPE (Solid Phase Extraction) SPE tube/cartridge HybridSPE-PLus 96-Well Plate, 50 mg/2 mL per well (575659-U) sample addition 300 µL of 1% formic acid acetonitile, 100 µL spiked plasma per well elution apply vacuum at 10 in Hg for 4 min column Ascentis Express F5, 10 cm x 2.1 mm, I...

HPLC Analysis of β-Blockers and β-Agonists in Urine and Wastewater on a C18 Column after SPE using SupelMIP® SPE-Beta-Receptor

sample preparation SPE (Solid Phase Extraction) sample/matrix horse urine or waste water spiked with 10 beta-agonists and beta-blockers at 1 ng/mL SPE tube/cartridge SupelMIP SPE - Beta-Receptor, 25 mg/10 mL (LRC) (53223-U) condition 1 mL acetonitrile; 1 mL DI water sample addition 1 mL washing 3 x ...