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LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC

instrument Agilent® 6530 Q-TOF LC/MS column SeQuant ZIC-cHILIC, 10 cm x 2.1 mm, 3.0 µm mobile phase [A] acetonitrile; [B] 50 mM ammonium acetate, pH 5.0 with acetic acid in water gradient 26 to 27% B in 10 min; to 27 to 35% B in 10 min; to 26% B in 0.1 min; hold at 26% B for 9.9 min flow rate 0.3 mL...