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GC Analysis of FAMEs in Cookies on SLB®-IL111 after SPE Fractionation using Discovery® Ag-Ion

sample preparation SPE (Solid Phase Extraction) sample/matrix 1 g of commercially purchased cookies was ground and subjected to acid digestion and alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 SPE tube/cartridge Discovery Ag-Ion SPE tubes, 750 mg/6 mL (54225...

GC Analysis of FAMEs in Rapeseed Oil (with Conjugated Linoleic Acid (CLA) FAME Isomers Added) on SP™-2560

column SP-2560, 200 m x 0.25 mm I.D., 0.20 µm (29688-U) oven 175 °C carrier gas hydrogen sample Mix of rapeseed oil FAME isomers and CLA FAME isomers injection 1µL, split 50:1 liner 4 mm I.D., split type, cup design inj. temp. 250 °C detector FID, 250 °C Analysis Note Four key conjugated linoleic ac...

GC Analysis of FAMEs in Walnuts on SP™-2560

column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056) oven 180 °C carrier gas helium, 1 mL/min sample ground walnuts prepared for analysis per AOCS Official Method Ce 1k-09 injection 1 µL, split 100:1 liner 4 mm ID FocusLiner w/taper inj. temp. 250 °C detector FID, 250 °C Analysis Note This applicat...

GCxGC Analysis of FAMEs in Human Plasma on Equity®-1 x SUPELCOWAX® 10, Non-Polar to Polar Strategy

column (1D) Equity 1, 30 m x 0.25 mm I.D., 0.25 µm (28406-U) column (2D) SUPELCOWAX 10, 0.95 m x 0.10 mm I.D., 0.10 µm (24343) oven 180 °C, 3 °C/min to 280 °C (10 min) oven (modulator) LMCS Everest longitudinally modulated cryogenic system, modulation period 6 sec carrier gas hydrogen sample human p...

GC Analysis of FAMEs in Rapeseed Oil on SLB®-IL100

column SLB-IL100, 30 m x 0.25 mm I.D., 0.20 µm (28884-U) oven 180 °C carrier gas helium, 30 cm/sec @ 180 °C sample rapeseed oil FAME mix, 5 mg/mL total FAMEs in methylene chloride injection 1 µL, 100:1 split liner 4 mm I.D., split, cup inj. temp. 250 °C detector FID, 250 °C Categories Analytical Chr...

GC Analysis of FAMEs in Rapeseed Oil (with Conjugated Linoleic Acid (CLA) FAME Isomers Added) on SLB®-IL111

column SLB-IL111, 200 m x 0.25 mm I.D., 0.20 µm (29689-U) oven 150 °C carrier gas hydrogen sample Mix of rapeseed oil FAME isomers and CLA FAME isomers injection 1µL, split 50:1 liner 4 mm I.D., split type, cup design inj. temp. 250 °C detector FID, 250 °C Analysis Note Four key conjugated linoleic ...

GC Analysis of FAMEs in Walnuts on SLB®-IL111

column SLB-IL111, 100 m x 0.25 mm I.D., 0.20 µm (29647-U) oven 168 °C carrier gas FID, 250 °C sample ground walnuts prepared for analysis per AOCS Official Method Ce 1k-09 injection 1 µL, split 100:1 liner 4 mm ID FocusLiner w/taper inj. temp. 250 °C detector FID, 250 °C Analysis Note This applicati...

GC Analysis of FAMEs in Popcorn Oil on SP™-2560 (75 m x 0.18 mm I.D., 0.14 μm) after SPE Fractionation using Discovery® Ag-Ion, Fast GC Analysis

sample preparation SPE (Solid Phase Extraction) sample/matrix 1 g of popcorn oil FAME's in Hexane SPE tube/cartridge Discovery Ag-Ion, 750 mg/6 mL (54225-U) condition 4 mL acetone and 4 mL hexane sample addition 1 mL of the hexane FAME extract elution 6 mL hexane:acetone (96:4, v/v) followed by 4 mL...

GC Analysis of a 38-Component FAME Mix on SLB®-IL111, 200 m Column

column SLB-IL111, 200 m x 0.25 mm I.D., 0.20 µm (29689-U) oven 100 °C (13 min), 4 °C/min to 240 °C (30 min) carrier gas hydrogen, 27 cm/sec sample Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME injection 1.0 µL, 50:1 split liner 4 mm I.D., split type, cup design inj. temp. 250 °C detector FI...

GC Analysis of Conjugated Linoleic Acid (CLA) FAME Isomers on SP™-2560

column SP-2560, 200 meters x 0.25mm ID x 0.2um (29688-U) oven 175 °C carrier gas hydrogen sample 4-component CLA FAME isomer mix, 0.5 mg/mL in methylene chloride injection 1µL, split 50:1 liner 4 mm I.D., split type, cup design inj. temp. 250 °C detector FID, 250 °C Analysis Note Four key conjugated...

GC Analysis of a 37-Component FAME Mix on SLB®-IL111

column SLB-IL111, 100 m x 0.25 mm I.D., 0.20 µm (29647-U) oven 140 °C (5 min), 8 °C/min to 180 °C, 5 °C/min to 260 °C carrier gas hydrogen, 40 cm/sec sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL, 100:1 split liner 4 mm I.D....

GCxGC Analysis of C4-C24 FAMEs on SLB®-5ms x SLB-IL60, Non-Polar to Polar Strategy

column (1D) SLB-5ms, 30 m x 0.25 mm I.D., 0.25 µm (28471-U) column (2D) SLB-IL60, 1 m x 0.10 mm I.D., 0.08 µm oven (1D) 40 °C (2 min), 4 °C/min to 270 °C (20 min) oven (2D) 55 °C (2 min), 4 °C/min to 280 °C (20 min) oven (modulator) temp. offset 30 °C, modulation period 3 sec carrier gas helium, 1.0...

GCxGC Analysis of a 37-Component FAME Mix on SLB®-5ms x SLB-IL60, Non-Polar to Polar Strategy

column (1D) SLB-5ms, 30 m x 0.25 mm I.D., 0.25 µm (28471-U) column (2D) SLB-IL60, 1 m x 0.10 mm I.D., 0.08 µm oven (1D) 40 °C (2 min), 4 °C/min to 270 °C (20 min) oven (2D) 55 C (2 min), 4 °C/min to 280 °C (20 min) oven (modulator) temp. offset 30 °C, modulation period 3 sec carrier gas helium, 1.0 ...

GC Analysis of a 37-Component FAME Mix on Omegawax®

column Omegawax 250, 30 m x 0.25 mm I.D., 0.25 µm (24136) oven 50 °C, 3.0 °C/min to 280 °C carrier gas helium, 40 cm/sec constant sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL50:1 split inj. temp. 240 °C detector FID, 280 °C...

GC Analysis of a 37-Component FAME Mix on SP™-2560, Slower Oven Ramp

column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056) oven 140 C (5 min) to 240 C (5 min) at 4 C/min carrier gas helium, 20 cm/sec sample volume 1 µL split 100:1, 260 C detector FID, 260 C Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs) Featured Industry Agric...

GC Analysis of Conjugated Linoleic Acid (CLA) FAME Isomers on SLB®-IL111

column SLB-IL111, 200 m x 0.25 mm I.D., 0.20 µm (29689-U) oven 150 °C carrier gas hydrogen sample 4-component CLA FAME isomer mix, 0.5 mg/mL in methylene chloride injection 1 µL1µL, split 50:1 liner 4 mm I.D., split type, cup design inj. temp. 250 °C detector FID, 250 °C Analysis Note Four key conju...

GC Analysis of a 38-Component FAME Mix on SP™-2560, 200 m Column

column SP-2560, 200 m x 0.25 mm I.D., 0.20 µm (29688-U) oven 100 °C (13 min), 4 °C/min to 240 °C (30 min) carrier gas hydrogen, 27 cm/sec sample Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME injection 1.0 µL, 50:1 split liner 4 mm I.D., split type, cup design inj. temp. 250 °C detector FID,...

GC Analysis of a 37-Component FAME Mix on SP™-2560

column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056) oven 140 °C (5 min), 8 °C/min to 180 °C, 4 °C/min to 210 °C, 20 °C/min to 250 °C (7 min) carrier gas hydrogen, 40 cm/sec sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL, 10...

GC Analysis of a 37-Component FAME Mix on Omegawax® (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

column Omegawax 100, 15 m x 0.10 mm I.D., 0.10 µm (23399-U) oven 140 °C, 40 °C/min to 280 °C (2 min) carrier gas hydrogen, 50 cm/sec constant sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 0.2 µL, 200:1 split liner 4 mm I.D., spli...

GC Analysis of a Grain Fatty Acid Methyl Ester (FAME) Mix on SP™-2560

column SP-2560, 100 m x 0.25 mm I.D., 0.20 mum film (24056) oven 140 C (5 min) to 240 C at 4 C/min carrier gas helium, 20 cm/sec injection 1 µLsplit 100:1 inj. temp. 260 °C detector FID, 260 C Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs) Featured Industry A...

GC Analysis of a 37-Component FAME Mix on Equity®-1 (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

column Equity-1, 15 m x 0.10 mm I.D., 0.10 µm (28039-U) oven 100 °C, 50 °C/min to 300 °C (1 min) carrier gas hydrogen, 50 cm/sec constant sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 0.2 µL, 200:1 split liner 4 mm I.D., split, c...

GC Analysis of a 37-Component FAME Mix on SP™-2560 (75 m x 0.18 mm I.D., 0.14 μm), Fast GC Analysis

column SP-2560, 75 m x 0.18 mm I.D., 0.14 µm (23348-U) oven 140 °C (5 min), 4 °C/min to 240 °C (2 min) carrier gas hydrogen, 40 cm/sec. @ 175 C sample 37 component FAME mix at concentrations listed in methylene chloride (47885-U) injection 1 µL, split 100:1 liner 4 mm I.D split, cup design inj. temp...

GC Analysis of a 37-Component FAME Mix on SLB®-IL100

column SLB-IL100, 30 m x 0.25 mm I.D., 0.20 µm (28884-U) oven 50 °C, 3.0 °C/min to 240 °C carrier gas helium, 40 cm/sec constant sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL50:1 split inj. temp. 240 °C detector FID, 240 °C ...

GC Analysis of cis/trans FAME Column Performance Mix on SP™-2560 per AOAC Method 2012.13

column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056) oven 60 °C (1 min), 15 °C/min to 165 °C (1 min), 2 °C/min to 225 °C (20 min) carrier gas helium, 0.8 mL/min sample cis/trans FAME Column Performance Mix (40495-U) injection 1 µL, 10:1 split liner 4 mm I.D., split/splitless type, wool packed singl...

GC Analysis of a 37-Component FAME Mix on SPB-PUFA

column SPB-PUFA, 30 m x 0.32 mm I.D., 0.20 µm (24323) oven 50 °C (2 min), 4 °C/min to 210 °C carrier gas helium, 25 cm/sec sample Supelco 37-Component FAME Mix (47885-U), analytes at concentrations indicated in methylene chloride injection 1 µL, 100:1 split inj. temp. 250 °C detector FID, 260 °C Cat...

GC Analysis of cis/trans FAME Column Performance Mix on SP™-2560 per AOAC Method Ce 1h-05

column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056) oven 180 °C carrier gas helium, 1 mL/min sample cis/trans FAME Column Performance Mix (40495-U) injection 1 µL, 100:1 split inj. temp. 250 °C detector FID, 250 °C Analysis Note Complex mixtures of cis/trans FAME isomers are difficult to separate....

GC Analysis of C18:0, C18:1, C18:2, and C18:3 FAME Isomers on SP™-2560

column SP-2560, 200 m x 0.25 mm I.D., 0.20 µm (29688-U) oven (175 °C) carrier gas hydrogen sample Mix of C18:0, C18:1 (from partially hydrogenated vegetable oil [PHVO]), C18:2, and C18:3 FAME isomers injection 1.0 µL, 50:1 split liner Split type, cup design inj. temp. 250 °C detector FID, 250 °C Ana...

GC Analysis of a 38-Component Fatty Acid Methyl Ester (FAME) Mix on SLB®-IL60, Comparison to a Polyethylene Glycol (PEG) Column

column SLB-IL60, 30 m x 0.25 mm I.D., 0.20 µm (29505-U) column PEG 3, 30 m x 0.25 mm I.D., 0.25 µm oven 170 °C, 1 °C/min to 225 °C carrier gas helium, 1.2 mL/min sample Supelco 37-Component FAME Mix (47885-U) + C22:5n3, in methylene chloride liner 4 mm I.D., split/splitless type, single taper wool p...

GC Analysis of C18:0, C18:1, C18:2, and C18:3 FAME Isomers on SLB®-IL111

column SLB-IL111, 200 m x 0.25 mm I.D., 0.20 µm (29689-U) oven 150 °C carrier gas hydrogen sample (Mix of C18:0, C18:1 (from partially hydrogenated vegetable oil [PHVO]), C18:2, and C18:3 FAME isomers) injection 1µL, 50:1 split liner Split type, cup design inj. temp. 250 °C detector FID, 250 °C Anal...

GC Analysis of cis/trans C18:1, C18:2, and C18:3 FAME Isomers on SP™-2560 (75 m x 0.18 mm I.D., 0.14 μm), Fast GC Analysis

column SP-2560, 75 m x 0.18 mm I.D., 0.14 µm (23348-U) oven 180 °C, isothermal carrier gas hydrogen, 25 cm/sec @ 180 °C sample mixture of C18:1, C18:2, and C18:3 FAMEs in methylene chloride injection 0.5 µL, split 100:1 liner 4 mm I.D. split, cup design inj. temp. 220 °C detector FID, 220 °C Categor...