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HPLC Column Fundamentals 1: Terms & Relationships [VIDEO]

This 4-part Supelco web seminar provides an introduction to how solutes can interact with columns containing porous particles (or stationary phases) to result in a chromatography separation. The efficiency, retention and selectivity terms in the resolution equation will be explained as well as how c...

Custom Peptide Library | PEPscreen®

Related Links • Peptide Portfolio • Quotation Request FAQ Peptide Library Stability Storage & Handling Guidelines Quotation Request Order Now Experience affordable and fast delivery of custom peptide libraries for target identification. The PEPscreen peptide library is configured in a convenient pla...

High Purity Inorganics

Aldrich-APL (AAPL) offers a complete line of the highest purity inorganic salts and materials for the micro and nanoelectronics market. Through proprietary processes, AAPL manufactures metal halide salts as anhydrous beads or powders. These ultra-high purity materials have total metals impurities 4N...

HPLC Column Fundamentals 4: Method Development [VIDEO]

This 4-part Supelco web seminar provides an introduction to how solutes can interact with columns containing porous particles (or stationary phases) to result in a chromatography separation. The efficiency, retention and selectivity terms in the resolution equation will be explained as well as how c...

Peptide Library Frequently Asked Questions (FAQ)

How are PEPscreen peptides synthesized? PEPscreen peptides are synthesized on proprietary, state-of-the-art robotic platforms utilizing optimized protocols based on Fmoc-chemistry. What are the capabilities of the synthesis platforms? Each platform can simultaneously synthesize hundreds of peptides....

Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum

Using HybridSPE®-Phospholipid, Ascentis® Express, and Other LC-MS Workflow Components The unique combination of column selectivity using Ascentis Express F5 and phospholipid removal using HybridSPE-PLus provides a robust and accurate LC-MS method for accurate and sensitive analysis of vitamin D meta...

HPLC Column Fundamentals 3: Stationary Phase Selectivity [VIDEO]

This 4-part Supelco web seminar provides an introduction to how solutes can interact with columns containing porous particles (or stationary phases) to result in a chromatography separation. The efficiency, retention and selectivity terms in the resolution equation will be explained as well as how c...

Custom Services - Standards, Packaging & CustomWare OEM

Sigma-Aldrich helps you to streamline reliable, efficient delivery of premium-grade testing components at the velocity essential for success. Our quality products and solutions allow you to work smarter, enabling the community a safe and healthier world. Organic Custom Standards • For environmental,...

Quick, Sensitive LC/MS/MS Analysis of Aflatoxins in Cannabis

Related Products • Cannabis Testing • Food & Beverage Analysis • Toxins (other than pesticide/drug residues) • Supel™ Tox SPE Cartridges Related Articles • Reporter US Volume 34.2 • Analysis of Residual Solvents in Hemp Extract by Headspace SPME-GC/MS • Headspace SPME GC/MS Analysis of Terpenes in C...

Biodiesel

Biodiesel is a renewable, alternative diesel fuel produced from vegetable oils, animal fats, or recycled restaurant grease. This non-toxic, biodegradable liquid fuel consists of mono-alkyl esters of long chain fatty acids (also known as fatty acid methyl esters, or FAMEs) and may be used alone or bl...

siRNA Frequently Asked Questions for Functional Genomics and RNAi

If you do not find the answer here in our FAQ (frequently asked questions), please send a request to sirnarequest@sial.com. General Duplex Design Experiment & Analysis in vivo Applications & N-TER Use Validation & Guarantee What is siRNA? siRNA (short [or small] interfering RNA) is a class of small ...

HPLC Column Fundamentals 2: Column Variables [VIDEO]

This 4-part Supelco web seminar provides an introduction to how solutes can interact with columns containing porous particles (or stationary phases) to result in a chromatography separation. The efficiency, retention and selectivity terms in the resolution equation will be explained as well as how c...

SILu™Prot Accurate Internal Standards in Quantitative MS

• We recently developed a novel collection of Stable Isotope-labeled (SIL) full length proteins designed to be used as mass spectrometry internal standards for quantitative proteomics. • These standards are spiked at the initial stage of the analytical workflow, thus significantly minimizing variati...

Scorpions® Probes

Scorpions probes are highly sensitive, sequence-specific, bi-labeled fluorescent probe/primer hybrids designed for qPCR. • SNP detection • Allele discrimination • Pathogen detection • Multiplexing • Viral load quantification • Gene expression analysis • Gene copy determination • Endpoint genotyping ...

Gas Chromatography | Supelco GC Columns and Accessories

Our full-line of capillary gas chromatography (GC and GC/MS) columns includes ionic liquid, MS-grade, Fast GC, GCxGC, chiral, and PLOT columns to serve multiple industries (including environmental, petroleum, chemical, food & beverage, and pharmaceutical). We also offer the most complete line of pac...

An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms

Related Products • Biopolymer Separations • BIOshell™ HPLC Columns • BIOshell™ Fused-Core® Columns • Tool for Mass Spectrometry Related Articles • Reporter US Volume 34.2 • High Resolution and High Efficiency Separations of mAbs and ADCs Using Proteomix HIC Columns • Improving the Chromatographic Se...

Analysis of NBOMe Hallucinogens from Urine

Related Articles • Reporter US Volume 33.3 (4 Mb PDF) • LC-MS of Illicit Bath Salts in Urine • Utilizing Novel SPME LC Tips for Forensic Screening of Drugs of Abuse in Urine Samples • SPME in the Analysis of Illicit Drugs • Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using ...

Bioethanol

Related Information • Biodiesel • Detection of Chloride and Sulfate with Dication and Trication Solutions Bioethanol is a renewable, alternative fuel traditionally produced by the yeast fermentation of sugar. When used as an oxygenated additive in gasoline to yield a cleaner-burning fuel, blends typ...

How to Select the Right Water Purification System for Your Lab

Related Products • Milli-Q® IQ 7003/05/10/15 Type-1 & Type-2 Water • Milli-Q® IQ 7000 Type-1 Water • Elix® Advantage • Milli-Q® HX 7000 Series Type-2 High-Throughput Water • Milli-Q® CLX 7000 Connected High-Throughput for Clinical Analyzers • SDS 500 - Water Storage, Protection & Distribution System...

Ultra-High Performance using Fused-Core® HPLC Columns [VIDEO]

This 28-minute Supelco HPLC web seminar discusses how to achieve ultra-high performance on any HPLC system using Ascentis® Express Fused-Core HPLC columns; also, how these columns help to reduce your solvent consumption. Seminar Abstract The web seminar covers the following points: • Introduction to...

Introduction | BioFiles Volume 5, Number 2 Protein Characterization & Detection

BioFiles Volume 5, Number 2 — Protein Characterization & Detection Download Biofiles v5 n2 (3.4 Mb PDF) Table of Contents • Introduction • Labeling of Proteins • X-ray Crystallography/Crystallization • MALDI Mass Spectrometry • Colorimetric Electrophoresis Stains • IEF Focusing • Western Blotting • ...

GC-MS Solvents

Related Categories • Solvent Center • GC Solvents • Gas Chromatography • Analytical Chromatography We have recently developed a new high purity solvents portfolio for your reliable GC mass spectrometry analysis. These solvents undergo rigorous purification procedures to assure high chemical purity. ...

Improving Product Safety Profiles

Post-translational modifications have been shown to affect the bioactivity, clearance rates, immunogenicity and safety profiles of therapeutic glycoproteins. For example anti- N-glycolylneuraminic acid (Neu5Gc or NGNA) antibodies in humans can interact with Neu5Gc sialylated therapeutic proteins (fo...

ABI3900 Synthesizer Columns with CUTAG CPG Frits

Proligo® presents the next generation support for reliable, cost-effective oligo synthesis without cross-contamination. A novel 0.2 μmol ready-to-use Universal ABI 3900 synthesizer column for the synthesis of top quality oligonucleotides has been developed. This new column contains a universal CUTAG...

APPI Dopants - Solvents and post-column additives for photo-ionization

• • Download PDF • R&D Chemist, LC/MS Applications rudolf.koehling@sial.com APPI(atmospheric pressure photoionization) dopants enable and enhance ionization in the APPI source. They include substances like toluene, acetone, anisole and chlorobenzene. High gas phase concentrations of the dopants are ...

Cost Reduction from Increased Throughput and Improved LC-MS Data Consistency

Phospholipids are major components of all cell membranes; and phospholipid contamination has been identified as one of the principal causes of ion-suppression when performing LC-MS analysis of small molecules in biological samples. They also buildup on analytical columns, elute unpredictably in down...

Protease Inhibitor Cocktails References

Our portfolio of protease inhibitor cocktails are frequently cited in peer-reviewed scientific literature, suggesting their superior quality and excellent track record. This page lists a sampling of those research papers. Other citations are available under the specific product pages for each of our...

Accurate Biological Testing for Amphetamine and Methamphetamine Abuse using Chiral HPLC and MS Detection

Denise Wallworth and Hugh Cramer, Supelco denise.wallworth@sial.com In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine. Methamphetamine and amphetamine, powerful CNS stimulants...

Increase Bioanalytical Assay Speed via Phospholipid Removal using HybridSPE™-Precipitation Technology

Craig Aurand and An Trinh an.trinh@sial.com One of the primary concerns when analyzing small molecules in biological samples via LC-MS/MS is ion-suppression. Ion-suppression is caused by one or more interfering components or species that co-elute with analyte(s) of interest during LC-MS analysis and...

Glycan Labeling

Glycobiology Analysis Manual Table of Contents GlycoProfile™ Products for Metabolic Glycan Labeling FLAG® Antibodies for Detection of FLAG-labeled Glycans GlycoProfile™ Labeling Kits Fluorophores Chromophores Additional Reagents for Reductive Amination Structural modifications of proteins are essent...