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827 matches found for sample_preparations

Analysis of Blood Alcohols on the SUPELCOWAX™ 10

Michael D. Buchanan and Robert F. Wallace mike.buchanan@sial.com Gas chromatographic analysis of blood alcohols is preceded by one of several sample introduction techniques: direct injection, headspace, or solid phase microextraction (SPME). Each of these techniques has distinct advantages over the ...

Performing a Purification of IgG Antibodies with rProtein A Sepharose Fast Flow

Related Articles • Automated Purification of Antibodies Using ÄKTA Chromatography Systems • Column Packing and Preparation for Affinity Chromatography of Antibodies • Converting from Flow Velocity to Volumetric Flow Rates • Desalting and Buffer Exchange for Affinity Chromatography of Antibodies • Ge...

Performing a Separation with Sephadex

Related Products • Column Packing and Preparation for Size Exclusion Chromatography • Packing a Column and Performing a Separation with Sephadex® LH-20 • Performing a Separation with Sephacryl® • Performing a Separation with Superdex® • Performing a Separation with Superose® • Sample Preparation for...

Analysis of Human Plasma Lipids Using sub-2 μm C18 UHPLC Column with MS Detection

Related Articles • Reporter US Volume 34.4 • Robustness of the Discovery® HS F5 HPLC Column in the Study of Metabolomics • Reaching the “Peak” of Recovery: Improving Antibody Separations with the Inclusion of Organic Alcohols • Rapid Determination of Protein Binding Affinity Using Solid Phase Microe...

Supelpak™-2SV Adsorbent Improves Environmental Sampling and Recovery of Difficult Semivolatile Compounds

Jim Walbridge jwalbridge@sial.com Supelco is please to announce the availability of its newest adsorbent, Supelpak-2SV. This new adsorbent is optimized for the sampling and analysis of semivolatile pollutants, dioxins, furans, polynuclear aromatic hydrocarbons (PAHs) and chlorinated pesticides. Supe...

Supelco expands SPME to Bioanalytical Applications

Key Features and Benefits • Direct sampling of biofluids • Easily automated for fast method development • Suitable for LC-MS, DESI, DART, and other systems Watch a brief video presentation on BioSPME - technology, benefits, and applications Technology Overview BioSPME Tips, based on SPME, is a fast ...

Pesticides & Herbicides - Chromatography and Sample Prep Literature from Supelco

Pub. Id. Subject Type Size (KB) No. of Pages T100758 Analyzing Chlorinated Pesticides by Packed Column and Capillary Column GC Bulletin 758 208 8 T100769 Monitoring Airborne Contaminants in Workplace Atmospheres, Using Sampling Devices and GC or HPLC Bulletin 769 345 12 T196900 Solid Phase Extractio...

STRboost™ Protocol for enhanced PCR with difficult samples

STRboost is a novel reagent that substantially enhances multiplex PCR amplification reactions used for human identification applications, resulting in improved accuracy, sensitivity and precision. Including STRboost in amplification reactions helps resolve stochastic effects typically associated wit...

SILu™PrEST – Isotope-labeled Multipeptide Standards for Quantitative Mass Spectrometry

Within the field of proteomics, mass spectrometry (MS) has become the method of choice for protein analysis.1 Using MS, thousands of proteins from a complex sample can be identified in a single run. Apart from enabling protein identification, MS has also become a key tool for protein quantification....

Analyzing Properties of Fluorescent Dyes Used for Labeling DNA in Microarray Experiments

BioFiles 2011, 6.3, 9. Dr. Jens Sobek, Catharine Aquino, Dr. Ralph Schlapbach, Functional Genomics Center Zurich, Zurich, Switzerland Cy3 and Cy5 are fluorescent dyes frequently used for microarray analysis. These two dyes have favorable properties, including relatively high fluorescence quantum yie...

Phenols - Chromatography and Sample Prep Literature from Supelco

Pub. Id. Subject Type Size (KB) No. of Pages T408127 Ascentis Express RP-Amide HPLC Columns Product Info. 536 2 T400130 The Quantitative Analysis of Semi-Volatile Organic Compounds on the MDN™-5S Capillary Column Product Info. 120 23 T409118 Ascentis Express HPLC Resource Guide Brochure 2,100 28 T10...

Sample Preparation in Ion Exchange Chromatography

Appendix 1, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007 Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, may reduce the need...

Proteomics and Protein Expression Product Guide - Most comprehensive protein research offering available.:

General Information (252 Kb PDF) Sample Preparation (4.89 Mb PDF) • Sample Prep Selection Guide • Extraction • Sample Fractionation & Enrichment • Post-Translation Modification • Organelle Enrichment • Additional Reagents Quantitation (479 Kb PDF) Expression Profiling (419 Kb PDF) Separation (1.71 M...

SPE Products for Mycotoxin Analysis [VIDEO]

Supelco introduces the Supel Tox line of SPE products for sample clean-up prior to mycotoxin analysis. As mycotoxin awareness and government regulations increase worldwide, allowable toxin levels continue to decrease. For quantitative mycotoxin analysis, HPLC and GC are among the most accurate and w...

AOCS Reference Mixes Available from Sigma-Aldrich

The American Oil Chemists’ Society (AOCS) found certain Fatty Acid Methyl Ester (FAME) mixes to be useful as reference standards when performing quantitative analyses of animal and vegetable oils. The compositions of these reference mixes are cited in AOCS Method Ce 1-62. Through its Supelco brand, ...

Frequently Asked Questions (FAQ) - Solid Phase Microextraction

Also on this page: Key Resources Q: What SPME fiber should I use to extract a class of analytes? Two factors should be considered: • Polarity of the analyte • Volatility and molecular size of the analyte Polar analytes are attracted to polar phases (e.g., polyacrylate and Carbowax coatings). Fibers ...

Analysis of Active Cannabis Compounds in Edible Food Products: Gummy Bears and Brownies

Introduction Potency testing in marijuana-infused edibles is a problematic task that analytical labs are facing due to the complexity of the involved matrices. Among the most popular matrices are gummy bear candies and brownies. According to one laboratory site, the concentration of active ingredien...

Everolimus-d4: An Internal Standard for Quantitation of Everolimus and Related Immunosuppressants by LCMS

Everloimus-d4 was synthesized and certified for use as an internal standard in LCMS applications. Use of the standard in LCMS applications was demonstrated by quantitation of an everolimus control sample against a calibration curve. The synthesis was developed from rapamycin by analogy to published ...

Enzymatic Method for Determining Urea

This assay protocol is suitable for the colorimetric detection of urea in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Urea Assay Kit (MAK006). Urea concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm) p...

Sample Preparation for Size Exclusion Chromatography

Appendix 3, Extracted from Size Exclusion Chromatography Principles and Methods (PDF) GE Healthcare, 2014 Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can redu...

Life Science

Our Life Science portfolio delivers products and services for life science research in academic and pharmaceutical environments. Our focus on genomic, proteomic and cellular analysis is supported by sample preparation, research reagents, intuitive detection platforms, and services to help customers ...

Choosing the Right Adsorbent for Your Thermal Desorption Gas Chromatography Applications [VIDEO]

This webinar provides an overview of adsorbents that are suitable for thermal desorption (TD), and the factors you need to consider when selecting an adsorbent. The presentation provides details about adsorbent mesh sizes, surface area, and temperature limits; and how these parameters influence the ...

Tools for Measuring Energy Expenditure

• • Download PDF • ISOTEC Stable Isotopes: Tools for Measuring Energy Expenditure (786 Kb PDF) D.A. Schoeller, Ph.D. Professor Department of Nutritional Sciences University of Wisconsin Madison, Wisconsin Water, a simple nutrient that is so ubiquitous in nature that it is easily forgotten and pushed...

The “Dioxin Prep System”: A Multi-Layer Silica Gel Column Connected to a Dual-Layer Reversible Carbon Column for Rapid Determinations of PCDD/FS*

Reference methods (Methods US EPA 23 or 1613 B; European method EN-1948) for the quantitative analysis of the seventeen toxic 2 ,3 ,7 , 8-PCDD/Fs involve successive clean-up steps on various chromatographic adsorbents (multi-layer silica, Florisil, alumina, activated carbon) which considerably incre...

Immunoprecipitation Techniques

Appendix 3, Extracted from Affinity Chromatography Vol. 1: Antibodies, GE Healthcare, 2016 Target proteins can be isolated and enriched from crude cell lysates by immunoprecipitation (also known as immunoaffinity or pull-down techniques). An antibody selected for its specificity is first affinity captur...

User Guide: Integrity Test Kit for small volume devices

This document describes how to use the Integrity Test Kit for small volume devices to integrity test non-vented and vented filter units with membrane surface areas of less than 15 cm2 (2.3 in2). Larger devices can be integrity tested using an Integritest® automated system. NOTE: The Integrity Test K...

SPME-Fast Fit Fiber Assemblies (FFA) and Accessories

SPME-Fast Fit Fiber Assemblies (FFA) Holder and Storage Devices • SPME-Diffusive Sampling Fiber Holder (DFH) for TWA Air Monitoring • SPME-FFA Field Sampler (FFA-FS) for sampling and storing under tough conditions • SPME Storage Containers (Conventional Fibers and FFA) Multi Fiber EXchanger System (...

Analysis of Bisphenol A in Food by Solid Phase Microextraction Using an Overcoated Fiber

Introduction Bisphenol A (BPA) is commonly used for food packaging applications such as polycarbonate bottles, and the linings of metal cans used for soups, juices, etc. It is a suspected endocrine disruptor, and thus low level, long term exposure as a result of migration into food from packaging ma...

D3561-11– Products and Materials Guide

Standard Test Method for Lithium, Potassium, and Sodium Ions in Brackish Water, Seawater, and Brines by Atomic Absorption Spectrophotometry This test method covers the determination of soluble lithium, potassium, and sodium ions in brackish water, seawater, and brines by atomic absorption spectropho...

Sample Preparation for MALDI-MS

In its current state, MALDI is primarily based on the laser desorption of solid matrix-analyte deposits. The technique suffers from some disadvantages such as low shot-to-shot reproducibility, short sample life time and strong dependence on the sample preparation method, figure 2. A few research gro...