UHPLC Analysis of Catecholamines on Ascentis® Express F5

UHPLC Analysis of Catecholamines on Ascentis® Express F5


column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 60 °C
mobile phase [A] water; [B] 100 mM ammonium acetate (pH 4.1 with acetic acid); [C] acetonitrile; (88:2:10, A:B:C)
flow rate 0.3 mL/min
pressure 1880 psi (130 bar)
injection Catecholamines 25 µg/mL in 90:10, water:methanol
detector UV, 250 nm


Analysis Note The analysis of catecholamines in a clinical setting is often for the diagnosis of pheochromocytoma (a type of adrenal tumor) in symptomatic people. The analysis shown here on Ascentis Express F5 provides rapid resolution of five common catecholamines.
Categories Analytical Chromatography, Clinical, Catecholamines, catecholamines
Featured Industry Clinical Research
Clinical Testing
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC


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