The highly variable regions within the 16S ribosomal RNA (rRNA) gene sequences are being exploited with PCR and metagenomic sequencing to characterize the microbial strains within the gastrointestinal environment. The 16S rRNA sequence is a useful marker: ubiquitous within the microbial population, functionally stable, and large enough to profile against bioinformatic databases. 16S rRNA sequencing is less cumbersome than DNA-DNA hybridization and has been used to ascertain species distribution within the intestinal microbiota. Quantitative PCR can be coupled with 16S rRNA species-specific primers to assess intestinal species population or used solely to determine the effectiveness of pathogenic colonization inhibition by probiotic treatments.
We offer a broad array of qPCR kits and probes for all detection chemistries and all instrument platforms. Researchers can simply choose between SYBR® Green and probe-based detection, then select from the following formats:
- Standard qPCR - kits for plate-based and tube-based instruments
- Capillary tube qPCR - kits for the Roche LightCycler® 2.0 & 1.0
- High-Throughput qPCR - kits with reference dye premixed into the ReadyMix. Compatible with most ABI™ instruments
- One-Step qRT-PCR - kits that include reverse transcriptase for streamlined quantification of RNA levels
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SYBR® Green Detection
Standard qPCR
Compatible Platforms: ABI instruments; Roche LightCycler 480; Bio-Rad/ MJ instruments; Stratagene instruments; Corbett Rotor-Gene™ 6000 and Rotor-Gene 3000; Eppendorf Mastercycler® ep realplex instruments
Capillary Tube qPCR
Compatible Platforms: Roche LightCycler 2.0 & 1.0
High Throughput qPCR
Compatible Platforms: ABI 7300, 7700, 7900, and StepOne ™
One-Step qRT-PCR
Compatible Platforms: ABI instruments; Roche LightCycler 480; Roche LightCycler 2.0 & 1.0; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor-Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler ep realplex instruments
| Name |
Usage |
Cat. No. |
| SYBR® Green Quantitative RT-PCR Kit |
1 kit sufficient for 100 50-μl PCR reactions |
QR0100-1KT |
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Probe-based Detection
Standard qPCR
Compatible Platforms: ABI instruments; Roche LightCycler 480; Bio- Rad/MJ instruments; Stratagene instruments; Corbett Rotor-Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler ep realplex instruments
High-Throughput qPCR
Compatible Platforms: ABI 7300, 7700, 7900, and StepOne
One-Step qRT-PCR
Compatible Platforms: ABI instruments; Roche LightCycler 480; Roche LightCycler 2.0 & 1.0; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor-Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler ep realplex instruments
| Name |
Usage |
Cat. No. |
| Quantitative RT-PCR ReadyMix™ |
1 kit sufficient for 100 50-μl PCR reactions |
QR0200-1KT |
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PCR Labware
We offer a broad range of labware optimized for PCR reactions: pipette tips, labtop coolers, films, foils, plates, thermocyclers, tubes, and workstations. The following tables contain a small selection of PCR Labware. For a complete listing of PCR equipment and supplies, visit our labware page.
Equipment
| Name |
Cat. No. |
| Hettich® MIKRO™ 120 Centrifuge |
Z720089-1EA |
| Labnet MultiGene™ Gradient Thermal Cycler, with 96 well block |
Z739472-1EA |
| Labnet MultiGene™ Gradient Thermal Cycler, with 60 well block |
Z739693-1EA |
| Labnet MultiGene™ Mini Thermal Cycler, with 24 x 0.2 ml block |
Z739928-1EA |
| Labnet MultiGene™ Mini Thermal Cycler, with 18 x 0.5 ml block |
Z723436-1EA |
| Labnet Prism™ R Refrigerated Microcentrifuge |
Z723878-1EA |
PCR Tubes
| Name |
Cat. No. |
| Agilent Technologies/Stratagene PCR microtubes |
Z376418-1PAK |
| Agilent Technologies/Stratagene PCR microtubes |
Z376426-1PAK |
| Agilent Technologies/Stratagene PCR microtubes, Caps for 0.2 mL strip tubes |
Z377910-1PAK |
| Corning® PCR tubes |
CLS6531-960EA |
| Corning® PCR tubes |
CLS6571-960EA |
| Corning® PCR tubes |
CLS6530-1000EA |
| Corning® Thermowell™ PCR tubes, flat caps, assorted colors |
CLS3744-1000EA |
| Corning® Thermowell™ PCR tubes, flat cap, clear |
CLS3745-1000EA |
| Corning® Thermowell™ PCR tubes, dome caps, clear |
CLS3747-1000EA |
| Corning® Thermowell™ PCR tubes, dome caps, assorted colors |
CLS3746-1000EA |
| Corning® Thermowell™ PCR tubes, flat cap, clear |
CLS3750-1000EA |
| Corning® Thermowell™ PCR tubes, flat cap, assorted colors |
CLS3749-1000EA |
| FlipTube™ microcentrifuge tubes, clear |
Z738824-500EA |
| FlipTube™ microcentrifuge tubes, yellow |
Z738921-500EA |
| FlipTube™ microcentrifuge tubes, green |
Z739030-500EA |
| FlipTube™ microcentrifuge tubes, red |
Z739154-500EA |
| FlipTube™ microcentrifuge tubes, blue |
Z739251-500EA |
| Greiner™ PCR tubes |
Z617687-1000EA |
| Greiner™ PCR tubes |
Z617717-1000EA |
| Greiner™ PCR tubes, cap strips |
Z617709-125EA |
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