Peptide Library Design and Calculator Tool

A complimentary design studio for generating different types of peptide libraries. Design overlapping peptide fragment libraries, alanine scanning libraries, truncation libraries and positional scanning libraries.



Choose the library type you wish to design by clicking one of the 4 buttons above.

Instruction guide for peptide library online design tool

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Overlapping Peptide Library – This tool allows you to generate overlapping peptide fragments to cover the full length of your protein of interest. You are able to define sequence parameters such as peptide length and number of amino acids offset. The designed sequences can be edited and exported to either word or Excel format.

Alanine Scanning Library – This tool allows you to systematically substitute each amino acid position on your peptide sequences with alanine- the smallest of the naturally occurring amino acids that maintains it chirality. Alanine mutagenesis can be a powerful tool for probing the effect of individual amino acids side chain to protein function and properties.

Truncation Library – This tool allows you to build your peptide library by systematically removing flanking amino acids to determine the minimum length required for optimum activity. Once the essential amino acid residues for protein activity are identified by alanine scanning, the direction of truncation can be selected around these residues, rather than removing residues from both ends of the peptide sequence.

Positional Scanning Library – This tool allows you to optimize the peptide lead sequence. A selected position on the lead peptide is replaced with different amino acid (natural or unnatural) to improve the peptide properties such as biological activity, enzyme stability and solubility. The substitution can be performed on a single or multiple positions on a peptide sequence. A peptide library constructed using positional scanning can be very informative in studying protein interfaces whether in epitope mapping or protein-protein interaction.